肿节风注射液对裸鼠人胃癌SGC-7901移植瘤的抑制及诱导细胞凋亡作用研究  

Inhibitory Action of Sarcandra Glabra Injection on the Growth of SGC-7901 Transplantation Tumor in Nude Mice and Its Induction on Apoptosis

作　　者：赵益[1] 孙有智[2] 陈奇[2] 

机构地区：[1]江西中医学院现代中药制剂教育部重点实验室,南昌市330004 [2]江西中医学院,南昌市330004

出　　处：《中国药房》2009年第6期412-415,共4页China Pharmacy

摘　　要：目的:研究肿节风注射液(ZJF)对裸鼠人胃癌SGC-7901移植瘤的抑制作用及诱导细胞凋亡作用。方法:建立裸鼠人胃癌SGC-7901移植瘤模型,分为空白、阳性对照和ZJF高、低剂量(3、1.5g·kg-1)组。给予ZJF14d后,测定胸腺、脾脏指数和外周红细胞、白细胞、血小板数目;计算抑瘤率;观察瘤组织超微细胞形态;检测细胞凋亡变化。结果:与空白组比较,胸腺、脾脏指数和外周血象都无显著变化;ZJF高、低剂量组对裸鼠人胃癌SGC-7901移植瘤的抑瘤率有极显著性差异,分别为42.8%、39.9%;给药后细胞形成一个或多个凋亡小体;ZJF高、低剂量组对瘤组织的凋亡率有极显著性差异,分别是(9.3±0.15)%、(8.18±0.08)%。结论:ZJF可促使细胞出现凋亡小体、诱导细胞凋亡,从而抑制裸鼠人胃癌SGC-7901移植瘤的生长,并对胸腺、脾脏、外周血象无显著影响。OBJECTIVE： To study the inhibitory action of Sarcandra glabra injection （ZJF） on tumor growth of SGC- 7901 transplantation tumor in nude mice and its induction on apoptosis. METHODS： SGC-7901 transplantation tumor nude mice model was established then the model mice were assigned to blank, positive control, ZJF high-dose and low-dose （3, 1.5 g · kg^ -1） groups. After treatment with ZJF for 14 days, the indexes of thymus and spleen, numbers of peripheral red blood cells, white cells and platelet were measured; the tumor inhibition rate was computed; the ultrastructure of tumor tissues was observed, and the apoptosis of implanted tumor cells was observed. RESULTS： As compared with blank group, no significant change was noted in the indexes of thymus and spleen and peripheral hemogram. The differences between high- dose ZJF group and low - dose ZJF group were greatly significant in both the inhibition rate of SGC - 7901 transplantation tumor （42.8% vs. 39.9% ） and the apoptotic rate of tumor tissues [（9.3 ± 0.15）% vs. （8.18 ±0.08）% ] .One or more apoptotic bodies formed after medication. CONCLUSION： ZJF can promote the presence of apoptotic body and induce apoptosis thus inhibiting the growth of SGC - 7901 transplantation tumor in nude mice; however, it had little impact on the thymus and spleen and peripheral hemogram.

关 键 词：肿节风注射液 胃肿瘤 裸鼠移植瘤 细胞凋亡 

分 类 号：R979.1[医药卫生—药品] R730.52[医药卫生—药学]