甘草中甘草酸和甘草苷的提取纯化工艺研究  

Study on the Extraction and Purification for Glycyrrhizic Acid and Liquiritin from Radix et Rhizoma Glycyrrhizae

作　　者：赵祎镭[1] 师清芝[1] 唐星[2] 

机构地区：[1]哈尔滨医科大学第一临床医院药学部,哈尔滨市150010 [2]沈阳药科大学药学院,沈阳市110016

出　　处：《中国药房》2009年第6期426-429,共4页China Pharmacy

摘　　要：目的:筛选甘草中有效成分的提取工艺,并纯化甘草酸和甘草苷粗品。方法:以甘草酸和甘草苷的含量和提取率为指标,对不同提取溶剂进行筛选;确定提取溶剂后,以氨醇浓度、溶剂量、提取次数为考察指标,用正交试验优选提取工艺。通过重结晶法纯化甘草酸粗品,有机溶剂法纯化甘草苷粗品。结果:最佳提取溶剂为氨性乙醇;最佳提取工艺为以5倍药材量的0.3%氨水的60%乙醇,加热回流提取4次,每次2h。纯化后的甘草酸纯度高于85%,总提取率43%;纯化后的甘草苷含量高于15%,总提取率67%。结论:该工艺简便可行,同时纯化得到甘草酸和甘草苷2种成分,可拓宽甘草的应用范围。OBJECTIVE ： To optimize the extraction of effective ingredients and purification for crude glycyrrhizic acid and liquiritin from Radix et Rhizoma Glycyrrhizae with different methods. METHODS： The contents and extraction percentages of glycyrrhizic acid and liquiritin were used as indexes to optimize the extraction solvent. Then the extraction process was optimized with orthogonal experiment taking the concentration of ammonium ethanol, the amount of solvent and the extracting times as indexes. Crude glycyrrhizic acid was purified with the method of recrystallization and crude liquiritin was purified with organic solvent method. RESULTS ：The optimal extracting solvent was ammonium ethanol. The optimum extraction conditions were as follows：extracting the solvent 4 times（2 h/time） by adding 5 times volume of 60% ethanol（containing 0.3% ammonia water） . The purity of glycyrrhizie acid was over 85% with total extraction rate at 43%, and content of liquiritin was above 15% with its total extraction rate at 67% . CONCLUSION： The method is simple and feasible and from which glycyrrhizic acid and liquiritin was been obtained,Radix et Rhizoma Glycyrrhizae can broaden the scope of the application.

关 键 词：甘草 甘草酸 甘草苷 提取 纯化 

分 类 号：R284.2[医药卫生—中药学] R943[医药卫生—中医学]