毛细管电泳高频电导法测定威灵仙中熊果酸的含量  

Determination of Ursolic Acid in Radix et Rhizoma Clematidis by Capillary Zone Electrophoresis with High Frequency Conductance Method

作　　者：刘基柱[1] 张锋[1] 宋粉云[1] 

机构地区：[1]广东药学院,广州市510224

出　　处：《中国药房》2009年第6期436-438,共3页China Pharmacy

基　　金：2005年度广东省自然科学基金项目(5002841)

摘　　要：目的:建立以毛细管区带电泳、高频电导法测定威灵仙中熊果酸含量的方法。方法:采用未涂层弹性融硅石英毛细管柱(60cm×75μmID,有效长度56cm),以1.2mmol·L-1三乙胺-HCl(pH10.60)、0.24mmol·L-1β-环糊精为运行缓冲液,分离电压为12.5kV,重力进样10s(高度20cm)。结果:熊果酸检测浓度在5.55～111.0mg·mL-1范围内与对照品同内标峰面积的比值呈良好的线性关系(r=0.9993);平均回收率为96.0%,RSD=1.39%(n=6)。结论:本法简便、准确、快速、重现性好,可用于威灵仙中熊果酸的含量测定。OBJECTIVE：To establish a capillary zone electrophoresis with high frequency conductance method for the determination of ursolic acid in Radix et Rhizoma Clematidis.METHODS： The separation of sample was performed on an uncoated fused silica capillary（60 cm× 75μm ID, effective length： 56 cm） with 1.2 mmol·L^- 1 triethylamine - HCI（pH 10.60） and 0.24 mmol· L^ 1 β- cyclodextrin as running buffer solution.The separation voltage was 12.5 kV and the gravity injection of sample was conducted for 10 s at a height of 20 cm.RESULTS： The calibration curve of ursolic acid was linear over the concentration range of 5.55-111.0 mg · mL^-1（r = 0.999 3） and the average recovery for ursolic acid was 96.0% （RSD = 1.39%, n = 6） . CONCLUSION： The method was proved to be simple, accurate, rapid and reproducible, and suitable for the quantitative determination of ursolic acid in Radix et Rhizoma Clematidis.

关 键 词：毛细管电泳法 高频电导 威灵仙 熊果酸 

分 类 号：R284.1[医药卫生—中药学] R927.2[医药卫生—中医学]