猪链球菌2型四川分离株毒力基因的检测及其序列分析  

Sequence Analysis and Virulence-related Genes Detection of Streptococcus suis 2 Isolated in Sichuan Province

作　　者：赵永刚[1] 王君玮[1] 王志亮[1] 赵云玲[1] 王清华[1] 张维[1] 陈义平[1] 刘华雷[1] 王华[2] 王伟伟[3] 田晓灵[1] 

机构地区：[1]中国动物卫生与流行病学中心国家外来动物疫病诊断中心,山东青岛266114 [2]扬州大学农学院,江苏扬州225600 [3]四川农业大学,四川成都610000

出　　处：《中国动物检疫》2009年第3期25-28,共4页China Animal Health Inspection

基　　金：国家"十五"攻关项目2003BA712A04-04

摘　　要：根据猪链球菌2型的MRP、EPF和SLY基因设计合成了四对引物,对我们分离鉴定的2005四川分离株进行毒力因子检测,并对阳性产物测序比较。结果以分离株为模板,获得了与预期结果一致的867bp(MRP)、572bp(EF)的2个片段,以及SLY的长度为1502bp目的片段(外引物)和1443bp的目的片段(内引物)。说明分离株SSsc0501为MRP+EF+SLY+,属于具有3种主要毒力因子的强毒株。经对PCR阳性产物测序及序列分析,结果其与P1/7株的核苷酸序列完全一致,与1933株的同源性达99.7%,仅在128位、217位、744位、1380位、1386位的5个核苷酸发生突变。在氨基酸水平上SSsc0501株与1933株的同源性达99.6%,有2个氨基酸发生替代。Virulence-related factors of SSsc0501, a strain of Streptococcus Suis serotype 2, isolated during the 2005 outbreak in Sichuan Province were detected and sequenced. Digested with EcoR I, the PCR product can produce a 867bp （MRP） and a 572bp （EF） DNA fragment as expected, as well as a 1502bp （exoprimer） and a 1443bp （innerprimer） DNA fragments of SLY gene. The result revealed that SSsc0501 was a high virulent strain with MRP^＋EF^＋SLY^＋.The PCR product from SSsc0501 strain was sequenced and nucleotide sequence analysis revealed that the sly gene from strain SSsc0501 had a high homology to strains 1933（99.7%） and P1/7 （100%）. Only 5 nucleotides developed mutants in site 128,217,744,1380 and 1386 of 1933 strain. Aminoacid analysis also demonstrated highly homologous（99.6%） with two aminoacids replaced.

关 键 词：猪链球菌2型 毒力基因 检测 序列分析 

分 类 号：S852.611[农业科学—基础兽医学]