禽淋巴细胞性白血病的诊断与病毒亚群鉴定  

Diagnosis of avian lymphoid leukosis and identification of virus subgroup

作　　者：罗明星[1] 周碧君[2] 李永明[2] 汪德生[2] 史开志[1] 杨峰 赵航[1] 袁圣[1] 付鸣剑[1] 

机构地区：[1]贵州大学动物科学院,贵州贵阳550025 [2]贵州大学动物疫病研究所,贵州贵阳550025 [3]贵阳市农业局兽防站,贵州贵阳550000

出　　处：《中国动物检疫》2009年第3期35-37,共3页China Animal Health Inspection

基　　金：贵阳市农业局科学技术计划项目项目合同号[2008动防-01];贵阳市农业局科学技术计划资助项目([2006]5001号);贵州省农业厅兽医科技计划项目(2008-06)

摘　　要：采用病理组织学方法对某商品蛋鸡场送检的发病鸡进行实验室诊断,在肿胀的肝、脾、肾和法氏囊组织切片中观察到成淋巴细胞增生病变。使用禽白血病A、B亚群抗体检测试剂盒对血清样本进行间接ELISA试验,抗体阳性率达到44.4%(12/27)。采集12只病死鸡的肝脏材料提取DNA样本,11份样本中扩增出ALV-A亚群特异性的病原核酸(691bp),检出率达到91.6%(11/12)。将扩增的目的基因克隆与测序,截取gp85基因片段可变区序列与ALV-A、B、C、D和E亚群参考毒株的相应序列进行比较,同源性分别达到89.0-89.6%、67.1-67.7%、69.1-70.1%、69.1-69.5%和70.9-72.0%。结果证实本次疫病是由ALV-A亚群病毒引起的淋巴细胞性白血病。Histopathological method was used for the diagnosis the diseased chickens in a commercial layer farm. The lymphoblast proliferative lesions were observed in tissue slides of swollen liver, spleen, kidney and Fabricius bursa.The serum samples were detected using avian leukosis virus antibody test kit-subgroup A,B （ALV-AB） and the antibody positive rate reached 44.4%（12/27）. The total DNA samples were extracted from liver materials of 12 dead chickens. A 691 bp fragment was amplified from 11 DNA samples,which was specific for the subgroup A of ALV and the detective rate accounted for 91.6%（11/12）.The amplified target genes were cloned and sequenced. The comparison was made against the variable region of gp85 gene with subgroup A,B,C,D and E of ALV. The homology reached 89.0-89.6% 、67.1-67.7% 、69.1-70.1% 、69.1-69.5%和 70.9-72.0% respectively. It was demonstrated that the lymploid leucosis was caused by subgroup A of ALV.

关 键 词：禽白血病病毒 A亚群 血清学检测 病理组织学检查 PCR鉴定 淋巴细胞性白血病 

分 类 号：S852.659.3[农业科学—基础兽医学]