新型固定化酶载体的合成及其功能  

Synthesis of new carrier of immobilized enzyme and its functionalization

作　　者：周桓[1] 张秋禹[1] 金凤[1] 

机构地区：[1]西北工业大学理学院应用化学系,陕西西安710129

出　　处：《化工进展》2009年第3期462-467,共6页Chemical Industry and Engineering Progress

摘　　要：通过悬浮聚合,以甲基丙烯酸缩水甘油酯(GMA)为功能单体,二甲基丙烯酸乙二醇酯(EGDMA)和二乙烯基苯(DVB)为交联剂,合成不同交联度的新型固定化酶载体。利用压汞仪分析测定了载体的孔结构(平均孔径14.1nm、比表面积达到112.286m2/g),研究了该新型载体在不同溶剂中的溶胀行为。通过对载体的功能化研究,发现载体分子链上的环氧基易于与胺基发生反应,并讨论了胺化树脂弱碱交换量,其在异丙醇中达到7.72mmol/g。最后通过与木瓜蛋白酶的固定化反应表明以多乙烯多胺为胺化剂制得的酶载体活性最高,测得为2144U/g。A series of new carriers with different degrees polymerization with glycidyl methacrylate （GMA） of crosslinking were synthesized by suspension as functional monomer, ethylene glycol dimethacrylate （EGDMA） and divinyl benzene （DVB） as cross-linking agents. Mercury porosi-metry was used to characterize the pore structure （average pore diameter and surface area reached 14.1 nm, 112.286 m^2/g, respectively）. The swelling behavior of carriers in different solvents was also studied. At the same time, the functionalization of carder was investigated, and the results showed that the carriers could react with amino group readily. The weak base exchange capacity, which reached 7.72 mmol/g in isopropanol was discussed. Finally, immobilization reaction of papain indicated that the activity of enzyme carriers was the highest in polyethylene polyamine, at a value of 2144 U/g.

关 键 词：悬浮聚合 GMA-EGDMA-DVB共聚物 载体 酶的固定化 孔结构 弱碱交换量 

分 类 号：Q814.2[生物学—生物工程]