酸味中药复方对糖尿病大鼠心肌组织晚期糖化终产物形成及其受体基因表达的影响  

Effects of Compounded Sour Medicinal Herbs on Deposition of AGEs and Gene Expression of RAGE in the Myocardial Tissue in Diabetic Rats

作　　者：朱德增[1] 殷桂香[1] 曹玉莉[1] 

机构地区：[1]中国人民解放军第二军医大学长海医院中医科,上海200433

出　　处：《中国中医药信息杂志》2009年第3期29-31,共3页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家自然科学基金(30572426)

摘　　要：目的以氨基胍(AG)为对照,观察酸味中药复方对2型糖尿病大鼠心肌组织晚期糖化终产物(AGEs)含量及受体(RAGE)基因表达的影响,探讨其防治糖尿病心肌病变的机制。方法采取腹腔注射链脲佐菌素(STZ)配合高热量饮食的方法建立糖尿病动物模型。成模后随机分为模型组、AG组、中药组。给药8周和12周后分别采用荧光法和荧光定量PCR法检测心肌组织AGEs含量和RAGE基因表达量。结果糖尿病大鼠心肌组织AGEs含量和RAGE基因表达量明显高于正常组(P<0.05);中药组和AG组AGEs含量和RAGE基因表达量明显低于模型组(P<0.05)。结论酸味中药复方可能通过抑制大鼠心肌组织AGEs形成和下调RAGE基因表达实现对高糖状态下心肌组织保护作用。Objective To investigate the possible mechanism of Compounded Sour Medicinal Herbs （CSMH） in preventing and amelioration type 2 diabetic meUitus （T2DM） rats chronic complication, by analyzing the effects of CSMH on the deposition of advanced glycation end products （AGEs） and gene expression of their receptor myocardial tissue in T2DM rats. Methods The rats were injected with streptozotocin （STZ） through peritoneum, and fed with high-fat and high-caloric diets to induce T2DM animal models. Then the rats were randomly divided into three groups： untreated group （model group）, AG treated group and CSMH treated group. The deposition of AGEs and gene expression of RAGE in the myocardial tissue of T2DM rats were detected separately by fluorescence and real time PCR method. Results The deposition of AGEs and gene expression of their receptor in myocardial tissue in control group were lower than that of T2DM rats （P〈0.05）. After treated, the deposition of AGEs and gene expression of their receptor in myocardial tissue in CSMH treated group were decreased compared with that of untreated group （P〈0.05）, and were equivalent to that of AG treated group. Conclusion CSMH can decrease the quantity of AGEs accumulation and down-regulated its receptor gene expression in the myocardial tissue in T2DM rats.

关 键 词：2型糖尿病 心肌组织 晚期糖化终产物 糖化终产物受体 基因表达 大鼠 

分 类 号：R289.11[医药卫生—方剂学]