微波消解-荧光猝灭法测定痕量铜的研究  

Fluoro-Quenching Method for the Determination of Trace Copper after Microwave Assisted Sample Digestion

作　　者：孙雪花[1] 

机构地区：[1]延安大学化学与化工学院,陕西延安716000

出　　处：《分析测试学报》2009年第1期120-122,共3页Journal of Instrumental Analysis

基　　金：陕西省教育厅科研基金资助项目(08JK489);陕西省延安大学校内专项基金资助项目(YDK2006-25)

摘　　要：利用微波消解前处理样品,在(CH2)6N4(六亚甲基四胺)-HCl介质中,溶液中的铜能与甲磺酸加替沙星形成稳定的络合物,使甲磺酸加替沙星的内源性荧光显著猝灭,据此建立了铜-甲磺酸加替沙星的荧光分析新方法。该体系的最大激发波长λex=365nm,最大发射波长λem=500nm,铜浓度在4.0×10-8～1.2×10-5mol/L范围内,与荧光猝灭程度成正比。检出限为1.2×10-8mol/L,回收率为97%～104%。该法可直接用于测定铜含量,结果令人满意。A fluorophotometry method was developed for the determination of trace copper in some tea samples after a microwave assisted sample preparation. In （CH2 ）6N4 -HCl medium, gatifloxacin mesilate showed a strong intrinic fluorescence at 500 nm by using excitation wavelengths of 365 nm. However, the addition of Cu （ Ⅱ ）would result in fluorescence quenching of gatifloxacin mesilate owing to the form of Cu （ Ⅱ ） - gatifloxacin mesilate ternary complex. The decrease of fluorescence in- tensity was linear with the concentration of Cu （ Ⅱ ） in the range of 4.0 ×10^8- 1.2 ×10^5 mol/L with a detection limit of 1.2 ×10^8 mol/L. The relative standard deviation of 5.0μmol · L-1 Cu （ Ⅱ ） was 1.29% （ n = 5 ）. The spiked recoveries of gatifloxacin mesilate ranged from 97% to 104%. The method was applied to the direct determination of copper in tea samples with satisfactory results.

关 键 词：微波消解 铜 甲磺酸加替沙星 荧光法 

分 类 号：O657.3[理学—分析化学] O614.121[理学—化学]