菊藤胶囊对高脂血症大鼠血脂及肝脏高密度脂蛋白受体基因表达的影响  

Effect of Chrysanthemum Vine Capsule on lipids and high density lipoprotein receptor liver gene expression in rats

作　　者：孙志峰[1] 李柱一[1] 刘玉林[2] 

机构地区：[1]第四军医大学唐都医院神经内科,陕西西安710038 [2]第四军医大学基础部药品研究中心,陕西西安710033

出　　处：《第四军医大学学报》2009年第4期320-323,共4页Journal of the Fourth Military Medical University

摘　　要：目的:观察菊藤胶囊对高脂血症大鼠血脂及肝脏高密度脂蛋白受体(SR-BI)基因表达的影响,从分子水平上探讨其作用机制,为其临床应用提供依据.方法:将60只雄性SD大鼠以普通饲料喂养1 wk后测定血清总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)和低密度脂蛋白胆固醇(LDL-C)基础值.根据TC水平随机分为:空白对照组、模型组、血脂康组及菊藤胶囊低、中、高剂量组,每组各10只.空白对照组普通饲料喂养,其余各组予高脂饲料;自由饮水.模型组灌胃生理盐水,血脂康组灌胃血脂康胶囊0.090 g/kg,菊藤胶囊低、中、高剂量组分别灌胃菊藤胶囊1.175,2.350,4.700 g/kg·1次/d,连续给药6 wk.采用酶法检测大鼠TC,TG,HDL-C和LDL-C;分别以RT-PCR技术和Western Blot法,对肝SR-BI在mRNA及蛋白水平进行检测.结果:模型组大鼠血TC,TG,LDL-C均显著高于空白对照组(P<0.05),HDL-C显著低于空白对照组(P<0.05),肝SR-BI mRNA及SR-BI蛋白含量与空白对照组比较显著降低(P<0.05).血脂康组及菊藤胶囊中、高剂量组大鼠血TC,TG,LDL-C均显著低于模型组(P<0.05),HDL-C显著高于模型组(P<0.05),菊藤胶囊高剂量组与血脂康组比较差异显著(P<0.05),血脂康组及菊藤胶囊中、高剂量组大鼠肝SR-BI mRNA及SR-BI蛋白含量与模型组比较显著升高(P<0.05).结论:菊藤胶囊可明显降低TC,TG,LDL-C,升高HDL-C,调节脂质代谢紊乱、治疗高脂血症,其作用机制可能与上调肝SR-BI的表达相关.AIM： To study the effect of Chrysanthemum Vine Capsule on blood fat of rats with hyperlipemia and on high density lipoprotein receptor liver gene expression. METHODS： Sixty SD rats were divided randomly into six groups： group A （normal group）, group B （model group ）, group C （Xue Zhi Kang group）, group D （low-dosage Chrysanthemum Vine Capsule group）, group E （utidium-dosage Chrysanthemum Vine Capsule group）, and group F （high-dosage Chrysanthemum Vine Capsule group）, with 10 rats in each group. Croup A was fed on normal forage, but other groups were fed on high fat forage. All the groups were allowed to drink water freely. Group A was fed on normal forage without intragastric administration, group B was given physiological saline 10 mL/（ kg·d） with intragastric adminis-tration, group C was given Xue Zhi Kang Capsule 0.09 g/ （kg·d） with intragastric administration, and group D, E and F received Chrysanthemum Vine Capsule 1.175, 2.350, 4.700 g/ （kg·d） respectively with intragastrie administration for six weeks. The levels of TC, TG HDL-C and LDL-C in the blood serum were examined and the changes of SR-BI gene expression in rat＇s liver were analyzed by RT-PCR and Western blot. RESULTS： Chrysanthemum Vine Capsule markedly decreased the levels of TC, TG and LDC-C in the blood serum, raised the level of HDL, and enhanced SR-BI gene expression in rats＇ liver. Remarkable effect of Chrysanthemum Vine Capsule was observed on hyperlipidemia. CONCLUSION： Chrysanthemum Vine Capsule is effective in treating hyperlipidemia possibly by enhancing SR-BI gene expression in rats＇ liver.

关 键 词：菊藤胶囊 高脂血症 高密度脂蛋白受体 基因表达 

分 类 号：R285.5[医药卫生—中药学] R589.2[医药卫生—中医学]