幽门螺杆菌oipA基因的开关状态及其与胃疾病的关系  

Helicobacter pylori oipA gene switch status and its association with gastic diseases

作　　者：李妮[1] 余菲菲[1] 林旭[1] 陈豪[1] 

机构地区：[1]福建医科大学病原生物学系福建省高校感染与肿瘤重点实验室,福州350004

出　　处：《中华消化杂志》2009年第2期122-125,共4页Chinese Journal of Digestion

摘　　要：目的了解幽门螺杆菌（Hp）oipA基因的开关状态并探讨其与胃疾病的关系。方法收集活检胃组织标本360份，对组织快速尿素酶实验阳性和部分阴性的活检标本进行Hp分离培养，分离菌经革兰染色镜检、尿素酶实验和16SrRNAPCR鉴定后，PCR扩增其oipA和cagA基因，测序分析oipA信号区的开关状态，分析功能性oipA与临床疾病之间的关联性。结果组织标本分离培养获得106株Hp，其中72株cagA基因阳性，87株oipA信号区基因阳性。经测序分析80株oipA基因信号区呈开放状态，为功能性oipA。87株Hp中有1株oipA基因信号区呈关闭状态，6株开关状态不能确定。在胃溃疡和萎缩性胃炎中，功能性oipA的检出率[分别为12／12和78．26％（18／23）]均显著高于cagA[分别为58．33％switch status和39．13％switch status]。结论功能性oipA与萎缩性胃炎和胃溃疡关系密切。Objective To understand the status of oipA gene of Helicobacter pylori （H. pylori） and to evaluate the relationship between oipA gene and digestive disease. Methods Biopsy gastric mucosa were obtained from 360 patients with digestive disease under gastroscopy. H. pylori was isolated from urease positive and some negative samples, and then identified by microscope, urease test and 16 S rRNA PCR. oipA and cagA genes of the isolates were amplified by PCR and the status of oipA signal region was analyzed after sequencing. The relationship between functional oipA gene of H. pylori and digestive disease was investigated. Results One hundred and six isolates were obtained. Seventy-two strains were positive for cagA gene and 87 were positive for oipA gene in signal region. Eighty strains were on open status in signal region called functional oipA gene and 1 was on off status. The status of other 6 strains were uncertain. The frequency of functional oipA in ulcer and atrophic gastritis were higher than that of eagA（100% and 78.26% vs 58.33% and 39. 13%）. Conclusion Functional oipA gene is closely related with ulcer and atrophic gastritis.

关 键 词：溃疡 胃炎 萎缩性 幽门螺杆菌 基因 

分 类 号：R686[医药卫生—骨科学]