山羊痘病毒培养及其细胞病变观察研究  

Culturing of Goat Pox Virus and Observation of Pathological Changes of the Cells

作　　者：李有文[1,2] 魏风[1] 郭志儒[1] 陈创夫[1] 

机构地区：[1]新疆石河子大学动物科技学院,新疆石河子832002 [2]新疆塔里木大学动物科技学院,新疆阿拉尔843300

出　　处：《安徽农业科学》2009年第3期1102-1104,共3页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学基金(30560109)

摘　　要：[目的]研究不同处理的细胞对病毒生长的影响。[方法]制备原代绵羊睾丸细胞,并用原代、传代和冻存3种处理的睾丸细胞对我国山羊痘病毒疫苗株分别进行培养,并用倒置显微镜和电子显微镜观察。[结果]病毒在3种处理的细胞上生长出现细胞病变的时间略有差异,并且细胞病变也不尽相同。[结论]不同处理的细胞出现的细胞病变不同。[ Objective] To study the effect of different treated cells on the virus. [ Method] Prepare primary sheep testicular cells, culture goat pox virus with primary, passage and frozen sheep testicular cells, respectively, and observe with inverted microscope and electron microscope. [ Result ] The time of CPE emergence and their varieties on the 3 kinds of treated cells are slightly different. [ Conclusion] CPE of the cells under different treatments are different.

关 键 词：山羊痘病毒 睾丸细胞 细胞病变 

分 类 号：S858.27[农业科学—临床兽医学]