人参水提物对β淀粉样蛋白诱导SH-SY5Y细胞凋亡的保护作用  

Neuroprotective Effects of Water Extract of Ginseng on SH-SY5Y Cell Apoptosis Induced by Amyloid β Peptide

作　　者：胡胜全[1,2] 余惠旻[2] 刘塔斯[1] 杨大坚[2] 陈新滋[2] 何翠君[2] 

机构地区：[1]湖南中医药大学药学院,湖南长沙410007 [2]广东省深圳市中药药学及分子药理学研究重点实验室,518057

出　　处：《时珍国医国药》2009年第2期257-259,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家“973”计划前期研究专项项目(No.2006CB708516)

摘　　要：目的研究人参水提物(water extracts of Ginseng,WEG)对β淀粉样蛋白(Aβ25-35)诱导SH-SY5Y细胞凋亡的保护作用。方法用Aβ25-35诱导体外培养的SH-SY5Y细胞凋亡,并用WEG进行保护。MTT法检测细胞存活率,Hoechst33258染色观察细胞形态的变化,流式细胞仪分析亚二倍体峰和DNA含量,检测细胞凋亡。结果Aβ25-3550μmol/L诱导SH-SY5Y细胞72 h后,镜下观察到细胞变圆,损伤并聚集,Hoechst33258染色可见明显的颗粒状和固缩状荧光,流式细胞仪检测到明显的亚二倍体峰,凋亡率达(37.30±0.69)%。而Aβ25-35和不同浓度的WEG(1,5,10,15 mg/ml)同时处理后,细胞形态明显改善,MTT值显著高于Aβ25-35处理组(P<0.01),流式细胞仪检测凋亡率分别降低到(14.70±3.18)%,(7.23±1.51)%,(6.14±0.40)%,(5.88±1.33)%,呈现剂量依赖关系。结论人参水提物对Aβ25-35诱导的SH-SY5Y细胞凋亡有显著的保护作用。Objective To study the neuroprotective effects of water extract of Ginseng on SH-SYSY cell apoptosis induced by Amyloid β peptide . Methods SH - SY5Y cells were treated with Aβ25-35 to induce apoptosis in vitro, water extract of Ginseng was added into the culture to test its neuroprotective effects. The cell viability was measured by MTTtest, the morphology of SH-SY5Y cells was observed by Hoechst33258 staining and DNA content by FCM. Results Treated by Aβ25-35μmol/L 72h later, SH- SY5Y cells turned rounder , aggregated and were positively stained with fluorochrome Hoechst 33258. Cells displayed a typical sub-diploid peak in flow cytometry ,and the percentage of apoptosis was （37.30 ± 0.69）% （P 〈 0.01 ）. When incubated with Aβ25-35μmol/L and different concentrations （1,5,10,15 mg · ml- 1 ） of WEG for 72 h, the characteristics of apoptosis as measured by FCM dose-dependently declined to （ 14.70 ± 3.18 ） %, （7.23 ± 1.51 ） %, （ 6.14 ±0.40） % and （5.88 ± 1.33 ） % respectively （P 〈 0.05 ）. Conclusion Water extract of Ginseng has marked neuroprotective effects on SH -SYSY cell apoptosis induced by Aβ25-35.

关 键 词：SH-SY5Y细胞 AΒ25-35 人参水提物 细胞凋亡 

分 类 号：R285.5[医药卫生—中药学]