绿升麻中菠萝蜜烷型三萜类成分研究  

Study on the Cycloartane Triterpenes of Actaea asiatiea

作　　者：卢滨[1] 范云双[2] 万定荣[3] 段宏泉[1] 

机构地区：[1]天津医科大学药学院,天津300070 [2]天津工业大学中空纤维膜过程教育部重点实验室,天津300160 [3]中南民族大学生命科学学院,湖北武汉430074

出　　处：《时珍国医国药》2009年第2期267-270,共4页Lishizhen Medicine and Materia Medica Research

基　　金：天津医科大学科研基金资助(No.2004XK32)

摘　　要：目的研究绿升麻中菠萝蜜烷型三萜皂苷类化学成分。方法以硅胶柱层析、凝胶层析等多种柱层析分离，制备高效液相色谱纯化，得到单体化合物，以各种有机波谱鉴定化合物结构。结果分离得到t5个菠萝蜜烷型三萜类皂苷化合物，鉴定为asiaticosideA（1）；asiaticosideB（2）；26-deoxyeimicifugoside（3）；cimicifugoside（23S，4）；25-甲氧基升麻醇-3—0-b—D-木糖苷（25—0-methyl—cimigenol-3—0-b—D—xylopyranoside，5）；25，2’-二乙酰升麻醇-3-O—B—D-木糖苷（25，2’-O—diacetylcimigenol-3-O—β——xylopyranoside，6）；24-乙酰升麻醇-3-O—β—-木糖苷（24-O—acetylshengmanol-3—0-β—D—xylopyranoside（23R，24R，7）；24—0-aeetyldahurinol-3-O—β——xylopyr-anoside（8）；23-乙酰升麻醇-3-O-8-D-木糖苷（23-O—aeetylshengmanol-3—0—β-D—xylopyranoside，9）；CimiracemosideM（10）；1213-acetoxy-313-hydroxy-24，25，26，27-tetranor—cycloart-7-ell-23，1613-olide-3-O-β-D—xylopyranoide（11）；1213-acetoxy-3β—hydroxy-24，25，26，27-tetranor—cycloartan-23，1613-olide-3—0-β—D—xylopyranoide（12）；CimiracemosideH（13）；Neocimic培enosidesB（14）；2’-O-乙酰基-25-脱水升麻醇-3-O—β—D-木糖苷（2’-O—acetyl-25-anhydrocimigenol-3—0～β-D—xylopyranoside，15）。结论菠萝蜜烷型三萜类皂苷化合物3～15均为首次从绿升麻中分离得到。Objective To isolate and elucidate the constituents of Actaea asiatiea. Methods Chemical constituents were isolated by repeated column chromatography （Toyopearl HW -40C and preparative HPLC） and their structures were elucidated on the basis of spectral data analysis. Results Fifteen cycloartane triterpenes were isolated from Actaea asiatiea, asiaticoside A, asiaticoside B, 26 - deoxycimicifugoside,Cimicifugoside（23S） ,25 - O - methyl - cimigenol - 3 - O - β— D - xylopyranoside,25,2＇ - O - diacetylcimigenol - 3 - O - β— D - xylopyranoside, 24 - O - acetylshengmanol - 3 - O - β— D - xylopyranoside （ 23 R, 24R ）, 24 - O - acetyldahurinol - 3 - O - β— D - xylopyranoside ,23 - O - acetylshengmanol - 3 - O - β— D - xylopyranoside, Cimiracemo- sideM, 12β— acetoxy - 3β— hydroxy - 24,25,26,27 - tetranor - cycloart - 7 - en - 23,16β— olide - 3 - O - β— D - xylopyrano- side, 12β— acetoxy - 3β— hydroxy - 24,25,26,27 - tetranor - cycloartan - 23,16β— olide - 3 - O - β— D - xylopyranoside, Cimiracemoside H, Neocimicigenosides B, 2＇ - O - acetyl - 25 - anhydroeimigenol - 3 - O - β— D - xylopyranoside. Conclusion Compound 3 - 15 were first isolated from Actaea asiatiea.

关 键 词：绿升麻 醋酸乙酯提取物 菠萝蜜烷型三萜皂苷 

分 类 号：R284.1[医药卫生—中药学]