和营安心方对慢性心衰大鼠心肌细胞凋亡的影响  

Effects of Heyinganxin Fang on Myocardial Apoptosis in Chronic Congestive Heart Failure Rats

作　　者：李永民[1] 王保和[1] 邢立强[2] 薄爱华[2] 吴迎爽[2] 周宁[2] 

机构地区：[1]天津中医药大学,天津300193 [2]河北北方学院,河北张家口075029

出　　处：《时珍国医国药》2009年第2期300-301,共2页Lishizhen Medicine and Materia Medica Research

基　　金：河北省科技厅指导课题(No.07276483);河北省张家口市科技攻关计划资助课题(No.0711046D-6)

摘　　要：目的观察和营安心方对慢性心衰大鼠心肌细胞凋亡的影响。方法以皮下大量注射异丙肾上腺素(ISO)的方法制备慢性心衰大鼠模型,随机分为模型对照组,卡托普利组,参麦注射液组,和营安心大、中、小剂量组。另设正常对照组,用TUNEL法检测心肌细胞凋亡,用免疫组化方法检测心肌细胞Bax,Bcl-2蛋白表达,计算Bcl-2/Bax比值。结果模型对照组TUNEL法心肌细胞凋亡严重,棕褐色细胞核明显增多,免疫组化图像分析Bax表达明显升高,Bcl-2,Bcl-2/Bax比值明显下降。与模型对照组比较,和营安心方组心肌细胞凋亡数量明显减少,Bax蛋白表达明显下降,Bcl-2,Bcl-2/Bax比值明显升高。结论和营安心方有减抑慢性心衰大鼠心肌细胞凋亡的作用。Objective To study the effects of Heyingansxin Fang on myocardial apoptosis in chronic congestive heart failure rats. Methods Animal model of heart failure was induced by subcutaneously injecting large dose of ISO and the rats were randomly di- vided into model group, captopril group, Shenmai injection group, high dosage of Heyinganxin Fang, middle dosage of Heyinganx- in Fang , small dosage of Heyinganxin Fang and normal group. Apoptosis was detected with TUNEL . The Bax and Bcl - 2 pro- teins of cardiomyocyte were detected with immunohistochemistry and Bel - 2/Bax ratio was calculated. Results Compared with normal group, apoptotic cells and brown cell nucleus increased significantly in model group, the expression of Bax increased but the expression of Bcl - 2 and Bcl - 2/Bax ratio decreased significantly. Compared with model group, apoptotic cells decreased sig- nificantly in Heyinganxin Fang group, expression of Bax decreased and the expression of Bcl -2 and Bcl -2/Bax ratio increased significantly. Conclusion Heyinganxin Fang has effect of inhibiting myocardial apoptosis in chronic congestive heart failure rats.

关 键 词：和营安心方 心力衰竭 细胞凋亡 血流动力学 

分 类 号：R285.5[医药卫生—中药学]