旱莲草总黄酮的提取及其体外抗氧化活性研究  

Study on Extraction of Flavone from Eclipta alba and its Antioxidation in vitro

作　　者：王雪梅[1] 张建胜[1] 戴云[1] 高云涛[1] 

机构地区：[1]云南民族大学化学与生物技术学院,云南昆明650031

出　　处：《时珍国医国药》2009年第2期356-358,共3页Lishizhen Medicine and Materia Medica Research

基　　金：云南省社会发展科技计划资助基金(No.2007B148M);云南省教育厅科学研究基金(No.06Y195C)

摘　　要：目的研究旱莲草总黄酮的提取及其抗氧化活性。方法利用Fentaon反应产生羟自由基.OH,光照核黄素产生超氧阴离子自由基O2-.,采用分光光度法研究旱莲草体外清除活性氧自由基的作用,用硫代巴比妥酸(TBA)分光光度法研究其对.OH诱发卵磷脂脂质过氧化和DNA氧化损伤的抑制作用。结果旱莲草总黄酮得率为3.96%,对.OH、O2-.的半清除率IC50为0.015 mg.ml-1,0.08 mg.ml-1;对脂质过氧化DNA氧化损伤的半抑制率为0.02 mg.ml-1,2.0μg.ml-1。结论旱莲草总黄酮能有效清除活性氧自由基,对卵磷脂脂质过氧化和DNA氧化损伤有显著抑制作用。Objective To study the extraction of flavone from Eclipta alba and its antioxidation function. Methods Scavenging effects of Eclipta alba on active oxygen species · OH and O generated by Fenton reaction and riboflavine photosensitization were 2 investigated by the means of spectrophotometry. The inhibitory effects of Eclipta alba on the lecithin lipid peroxidation and damage of DNA chain induced by hydroxyl radical were observed by thiobarbit uric acid spectrophotometry. Results Total flavonoids in Eclipta alba was 3.96% ; the semi -clearance rate of · OH and O - was 0. 015 mg · ml-l and 0.08 mg· ml-1 ; the semi -inhi- 2 bition rate of Lecithin lipid peroxidation and oxidation damage of DNA was 0.02 mg · ml -1 and 2.0 μg· ml -l. Conclusion E- clipta alba can scavenge active oxygen efficiently. It can also significantly inhibit lipid peroxidation and damage of DNA by hy- droxyl radical.

关 键 词：旱莲草 总黄酮 抗氧化 

分 类 号：R284.2[医药卫生—中药学]