多序岩黄芪根中总黄酮超声提取工艺研究  

Study on Ultrasonic Extraction Technics of Total Flavonoids in Hedysarum polybotrys

作　　者：海力茜.陶尔大洪 帕丽达[1] 韩加[2] 何坚[1] 

机构地区：[1]新疆医科大学药学院,新疆乌鲁木齐830054 [2]新疆医科大学公共卫生学院,新疆乌鲁木齐830054

出　　处：《时珍国医国药》2009年第2期359-360,共2页Lishizhen Medicine and Materia Medica Research

基　　金：新疆维吾尔自治区少数民族科技骨干特培专项基金(No.2003-4)

摘　　要：目的确定多序岩黄芪中总黄酮的优选超声提取工艺。方法采用正交实验法,考察溶剂量、提取次数、超声时间、溶剂浓度对提取率的影响,以芦丁为标准品,用紫外分光光度标准曲线法测定含量。结果所考察的因素中,多序岩黄芪总黄酮的提取影响程度为:溶剂浓度>提取次数>溶剂量>超声时间,即乙醇浓度70%,提取时间20 min,体积20 ml,提取次数为3次。结论超声乙醇提取法最佳工艺组合A3B2C1D3,可为利用多序岩黄芪工业生产黄酮类药用成分提供科学依据。Objective To optimize the ultrasonic extraction condition for flavonoids from Hedysarum polybotrys. Methods The optimal conditions, such as ethanol concentration, ultrasonic time, solvent quanity and extraction times were determined by orthog- onal design. Results The order of factors to affect the flavonoid extraction was ethanol concentration 〉 extraction times 〉 solvent quanity 〉 uhrasonice time. Conclusion The optimum ultrasonic extraction is ： A3 B3 C3 D3.

关 键 词：多序岩黄芪 总黄酮 正交实验法 

分 类 号：R284.2[医药卫生—中药学]