重组人FLT3配基对人脐血CD_(34)^+细胞的体外扩增作用  

Effect of recombinant human FLT3 ligand (rhFL) on in vitro expansion of human cord blood CD + 34 cells

作　　者：周生[1,2] 毛宁[1,2] 赵明[1,2] 潘凌亚[1,2] 王宜强 杜德林[1,2] 唐佩弦[1,2] 

机构地区：[1]军事医学科学院基础医学研究所 [2]北京协和医院妇产科

出　　处：《中华医学杂志》1998年第1期37-39,共3页National Medical Journal of China

基　　金：国家863高科学技术重点课题;国家自然科学基金

摘　　要：目的观察重组人ＦＬ（ｒｈＦＬ）对脐血ＣＤ＋３４细胞的体外扩增作用。方法用磁性细胞分离仪富集人脐血ＣＤ＋３４细胞。在ＣＤ＋３４细胞体外液体培养中加入不同的细胞因子，在不同时间取样测试ＣＤ＋３４细胞阳性百分率、晚期造血祖细胞（粒细胞巨噬细胞集落生成单位及红系爆增式集落形成单位）并计数细胞总数。结果用磁性细胞分离仪可快速富集脐血ＣＤ＋３４细胞，纯度高达８０％以上。ｒｈＦＬ可协同白细胞介素（ＩＬ）－３、ＩＬ－６、粒－巨噬细胞集落刺激因子及促红细胞生成素促进ＣＤ＋３４细胞总数在７天时扩增３．４倍，无ｒｈＦＬ的对照组ＣＤ＋３４细胞只扩增１．５倍，但对晚期造血祖细胞的扩增无明显协同作用。结论用排除法证实ｒｈＦＬ主要扩增了早期造血祖细胞。Objective To determine the effects of recombinant human FLT3 ligand (rhFL) on in vitro expansion of human cord blood CD + 34 cells. Methods Human cord blood CD + 34 cells were enriched by using a magnetic cell isolation system. CD + 34 cells were incubated in liquid culture in the presence of different cytokines. The total number of all cells and the number of CD + 34 cells and hematopoietic progenitor cells (CFU GM and BFU E) were counted at various time intervals. Results Human cord blood CD + 34 cells could be enriched to the purity of more than 80%. The number of CD + 34 cells increased 3.4 folds in the presence of rhFL+IL3+IL6+GMCSF+EPO in seven days, while in the control group without rhFL, it increased 1.5 folds. The difference of the number of more matured hematopoietic progenitor cells in the CD + 34 cell population between the control group and the rhFL group was not significant. Conclusion Only the primitive hematopoietic progenitor cells are expanded by rhFL according to the results obtained in the exclusion method.

关 键 词：rhFL 脐血 CD34^+细胞 抗的 体外扩增 

分 类 号：R457.9[医药卫生—治疗学]