线栓法致大鼠局灶脑缺血模型的评价及思考  

Evaluation and Reflection on the Suture-Occluded Method of the Model of Focal Cereberal Ischemia in Rats

作　　者：陈小睿[1] 孟宪丽[1] 孟保华[1] 李佳川[1] 

机构地区：[1]成都中医药大学,四川成都611130

出　　处：《时珍国医国药》2009年第2期424-425,427,共3页Lishizhen Medicine and Materia Medica Research

摘　　要：目的基于实验经验的总结,重点介绍线栓法致大鼠局灶性脑缺血模型的制作方法及操作程序,为建立较为稳定的脑缺血模型提供参考。方法采用线栓自颈总动脉插入,经颈内动脉阻断大脑中动脉以造成局灶性脑缺血模型,测定各组脑组织含水率及梗塞体积比。结果模型组脑组织含水率及脑梗塞体积比明显高于假手术组;阳性药尼莫地平能明显减轻线栓模型损伤引起的脑组织缺血,降低脑含水率和脑梗塞体积。结论通过对线栓法模型进行改良,可有效缩短造模时程,减少手术损伤,提高再灌注后存活率,使造模效果趋于稳定。Objective Based on the summary of the experiences from experiments, the method for model building and the details of manipulation of the model of focal cerebral ischemia by suture - occluded in rats were emphasized in this article in order to build steadier models. Methods The suture was inserted via CCA into ICA to block MCA for building the MCAO model of rats and then the water ratio and the percentage of the infarcted volume of the brain tissue were measured. Results The water ratio and the percentage of the infarct volume of the model group were significantly higher than those in sham operated group. As the posi- tive comparison, Nimodipine could significantly relieve the impairment caused by MCAO and decrease the water ratio and the per- centage of the infarct volume. Conclusion The suture -occluded method shorten the time, relieve the impairment in OPS, heighten the livalility after reperfusion and the model is steady.

关 键 词：线栓法 脑缺血 动物模型 

分 类 号：R285.5[医药卫生—中药学]