大鼠骨髓内破骨细胞样细胞形成的实验观察  

An experimental study of the formation of osteoclast like cell in rat bone marrow culture.

作　　者：樊继援[1] 尹潍[1] 曹平[1] 庞智玲 邱明才[1] 

机构地区：[1]天津医科大学总医院内分泌研究所

出　　处：《中华医学杂志》1998年第1期56-58,共3页National Medical Journal of China

摘　　要：目的建立大鼠骨髓中破骨细胞样细胞（ＯＬＣ）形成的培养方法，观察１，２５（ＯＨ）２Ｄ３和成骨细胞对ＯＬＣ形成的影响。方法Ａ组，单纯骨髓培养作对照；Ｂ组，骨髓和成骨细胞（来自新生大鼠颅骨）共同培养；Ｃ组，单纯骨髓培养加１，以２５（ＯＨ）２Ｄ３（终浓度１０－８ｍｏｌ）；Ｄ组，骨髓细胞和成骨细胞加１，２５（ＯＨ）２Ｄ３共同培养。培养７天后进行抗酒石酸酸性磷酸酶（ＴＲＡＣＰ）（＋）多核细胞作为ＯＬＣ的计数、细胞ＴＲＡＣＰ测定和骨片扫描电镜观察。结果骨髓培养开始各组未见ＯＬＣ。培养后Ａ组仍无ＯＬＣ，Ｂ组、Ｃ组偶见少量ＯＬＣ，而Ｄ组多于Ｂ组和Ｃ组（Ｐ＜０．０１）。Ａ组细胞ＴＲＡＣＰ低于其它三组，Ｂ组和Ｃ组低于Ｄ组（Ｐ＜０．０１）。Ｄ组骨片可见吸收陷窝形成，其它三组未发现。结论骨髓培养中ＯＬＣ的形成需要１，２５（ＯＨ）２Ｄ３存在，骨髓与成骨细胞共同培养对ＯＬＣ形成有明显促进作用。Objectives To set up a culture method of osteoclast like cell (OLC) formation in rat bone marrow and to investigate the effects of 1,25(OH) 2D 3 and osteoblasts on the formation of OLC.Methods The culture of bone marrow from 4 week old rats were studied for the formation of OLC. The culture was divided into four groups: group A, only bone marrow culture as control; group B, bone marrow and osteoblast (from the crania of newborn rats) coculture; group C, bone marrow culture with 1,25(OH) 2D 3 (final concentration 10 -8 mol/L); group D, bone marrow and osteoblast coculture with 1,25(OH) 2D 3.After 7 days of culture, the number of OLC which was recognized as tartrate resistant acid phosphatase (TRACP) (+) multinucleated cell, the cellular TRACP and the resorption pits on bone slice were examined. Results No OLC was present at the beginning of the bone marrow culture in any groups. OLC still failed to be detected in group A by the end of culture, and a few of OLCs could be found out in group B and C. The number of OLC in group D was more than that in the other groups ( P <0.01). The cellular TRACP in group A was lower than that in the other groups, and the cellular TRACP activity in group B and C was lower than that in group D ( P <0.01). In group D, several smaller resorption pits on The bone slice were observed.Conclusion The formation of OLC requires 1,25(OH) 2D 3 and coculture with osteoblasts favors the formation of OLC in bone marrow culture.

关 键 词：破骨细胞 骨髓内 细胞形成 OLC 

分 类 号：R329－33[医药卫生—人体解剖和组织胚胎学] R68[医药卫生—基础医学]