分子印迹-后化学发光法测定盐酸环丙沙星  

A molecular imprinting-chemiluminescence method for deternination of ciprofloxacin hydrochloride

作　　者：刘永明[1] 刘振波[1] 徐淑周[1] 

机构地区：[1]烟台大学化学生物理工学院,烟台264005

出　　处：《分析试验室》2009年第3期47-50,共4页Chinese Journal of Analysis Laboratory

基　　金：山东省教育厅(J06060)项目资助

摘　　要：以α-甲基丙烯酸为功能单体、乙二醇二甲基丙烯酸酯为交联剂合成了盐酸环丙沙星分子印迹聚合物,并制备了在线富集发光流通柱。建立了环丙沙星-Ce(Ⅳ)-亚硫酸体系的分子印迹-化学发光分析法。盐酸环丙沙星浓度在5×10-7～5×10-5mol/L范围内与发光强度呈线性关系,方法的检出限为5.2×10-8mol/L。方法已用于尿与鲅鱼样品中环丙沙星的检测。Molecularly imprinted polymer of ciprofloxacin hydrochloride was prepared by using methacrylic acid as the functional monomer and ethylene glycol dimethacrylate as the cross-linker. Using prepared MIP as the recognition material and ciprofloxacin-Ce^4＋-SO3^-2 chemiluminescence system, a molecular imprinting-chemiluminescence method was established for online concentrating and determining ciprofloxacin hydrochloride. The linear range of the method was 5.0×10^-7 - 5.0 ×10^-5 mol/L. The detection limit was 5.2 ×10^-8 mol/L, The method has been applied to the determination of eiprofloxaein hydrochloride in fish and urine samples with satisfactory results.

关 键 词：分子印迹 环丙沙星 化学发光 鲅鱼 

分 类 号：O657.3[理学—分析化学]