南林895杨抗旱耐盐基因DREB1C的转化  

Transformation of Drought and Salt Resistant Gene(DREB1C) in Populus×euramericana cv. Nanlin 895

作　　者：杨春霞[1,2] 李火根[1] 程强[1] 陈英[1] 

机构地区：[1]南京林业大学林木遗传与生物技术省部共建教育部重点实验室,南京210037 [2]江西省林业科学院,南昌330032

出　　处：《林业科学》2009年第2期17-21,共5页Scientia Silvae Sinicae

基　　金：国家自然科学基金(30471406);江苏省高技术研究项目(BG2003306)

摘　　要：以美洲黑杨杂种优良无性系南林895杨为转基因受体材料,利用农杆菌介导法转化与逆境胁迫相关的DREB1C基因。经潮霉素筛选,共获得80株抗性植株,经PCR及RT-PCR检测有30株抗性植株呈阳性,初步证明DREB1C基因已整合到南林895杨基因组中。抗性试验结果初步表明,转基因植株的抗旱、耐盐性均有所提高。An elite hybrid clone of Populus × euramericana cv. Nanlin895 was used as the receptor material for transgenic transformation, and Agrobacterium-mediated method was applied to introduce DREB1C gene encoding a transcription factor related with stresses. A total 80 resistant plants were gained via strictly selection by hygromycin. Among them 30 positive transgenic plants were verified by PCR and real-time PCR analysis. It is indicated that DREBIC gene was probably integrated into genome of Nanlin895. Furthermore, results of resistant experiment showed that resistance of transgenic plants to drought and salt was stronger than non-transgenic plants.

关 键 词：南林895杨 DREB1C基因 遗传转化 抗旱耐盐性 

分 类 号：S718.46[农业科学—林学] Q943.2[生物学—植物学]