异养硝化．好氧反硝化菌株DN1．2的脱氮特性研究  

Characteristics of heterotrophic nitrification and aerobic denitrification strain DN1.2

作　　者：黄钧[1] 杨航[1] 牟丽娉[1] 李毅军[1] 

机构地区：[1]中国科学院成都生物研究所成都610041

出　　处：《高技术通讯》2009年第2期213-218,共6页Chinese High Technology Letters

基　　金：863计划(2006AA06Z330)资助项目

摘　　要：对一株具有异养硝化-好氧反硝化能力的恶臭假单胞菌Pseudomonas putida DN1.2进行了研究,初步探讨了不同碳源种类、碳氮比、pH值、温度、氨氮质量浓度对DN1.2菌株脱氮作用的影响。结果表明,该菌在异养硝化过程中能同时去除化学需氧量(COD)和氨氮,并且不积累硝酸盐和亚硝酸盐。碳氮比是影响其脱氮效果的重要因素。不同碳源种类下菌株的脱氮能力按大小排序为:乙酸盐>葡萄糖>柠檬酸盐>甘油。脱除氨氮和COD的最适初始pH为7.0～7.5,最适温度为30～34℃。在菌株DN1.2转化的氨氮中有超过50%的部分被完全从水体中去除,细胞对氨氮的同化率为38.5%。The performance of a strain of Pseudomonas put/da named DN 1.2 with the ability to conduct both heterotrophic nitrification and aerobic denitrification was studied. The effects of different carbon sources, C/N, pH, temperature and molar concentration of ammonia on the strain DN1.2 were detected. The results show that the strain DN1.2 has the capability of removing ammonia and COD（ chemical oxygen demand） synchronously without accumulation of nitrate or nitrite.C/N is a key factor affecting denitrification. The comparison of the ammonia removal ability of the strain DN1.2 utilizing different carbon sources can be shown as acetate 〉 glucose 〉 citrate 〉 glucerol. The most suitable conditions for the strain DN1.2 to remove ammonia and COD are pH 7.0 - 7.5 and 30 - 34℃. There is over 50% of the total removed ammoniathat can be wiped off the water system. The ammonia assimilation rate of the cell is about 38.5 %.

关 键 词：异养硝化 好氧反硝化 恶臭假单胞菌DN1.2 

分 类 号：X703[环境科学与工程—环境工程]