HPLC法测定肋柱花中木犀草素的含量  

Determination of luteolin in Herba Lomatogonii by HPLC

作　　者：王秀梅[1] 巴根那[1] 白明纲[1] 

机构地区：[1]内蒙古民族大学蒙医药学院

出　　处：《中国民族医药杂志》2009年第1期38-39,共2页Journal of Medicine and Pharmacy of Chinese Minorities

摘　　要：目的：建立高效液相色谱（HVLC）法测定肋柱花中木犀草素含量的方法。方法：采用高效液相色谱法。色谱柱：Dia—monsilC18（250mm×4．6mm，5μm）；流动相：甲醇-0．4％磷酸溶液（55：45）；流速：1．0ml·min^-1；检测波长：350nm；柱温：35℃。结果：木犀草素对照品在0．052μg-0．312μg范围内，与峰面积具有良好的线性关系，r=0．9999；平均回收率为98．13％、RSD为1．04％（n=6）。结论：该方法简便、快捷、准确、重复性好，可用于肋柱花中木犀草素的含量测定。objective： To establish a method for the determination of luteolin in Mongolian medicine Lomatonium rataturn （L.） Fries by HPLC. Method： Determining the luteolin in Mongolian medicine Lomatonium rotatum （L.） Fries by ttPLC on Diamonsil C18（250mm×4.6mm,5μm） with Methyl- 0.4% Phosphoric acid （55：45 v/v） as a mobile phase and the flow rate is 1.0 ml·min^-1, the detective wavelength is 350 nm, the column temperature is 35℃ .Resuits ： The good linear relationship of Luteolin is abtained between 0.052μg - 0. 312μg, r = 10000. The average recovery is 98.13%, RSD = 1.04% （n = 6）. Conclusion： The method is simple, rapid, accurate, good re - appearance, and can be used for determining the content of Luteolin in Mongolian medicine Herba Lomatogonii.

关 键 词：蒙药 肋柱花 木犀草素 HPLC 

分 类 号：R291.2[医药卫生—民族医学]