二黄方对EAE大鼠细胞因子及Th1/Th2平衡的影响  

Influences of Erhuangfang on Cytokines and Balance of Th1/Th2 in EAE Rats

作　　者：周莉[1] 樊永平[1] 王蕾[2] 龚海洋[1] 

机构地区：[1]首都医科大学附属北京天坛医院中医科 [2]首都医科大学中医药学院

出　　处：《首都医科大学学报》2009年第1期20-23,共4页Journal of Capital Medical University

基　　金：国家自然科学基金(30640069);北京市自然科学基金(7062022);北京市中医药重点学科基金(2004京中重Ⅳ14)资助项目~~

摘　　要：目的探讨二黄方对实验性自身免疫性脑脊髓炎(experimental autoimmune encephalomyelitis,EAE)大鼠不同时间点血浆IFN-γ、IL-4及Th1/Th2(IFN-γ/IL-4)平衡的影响。方法用髓鞘碱性蛋白(myelin basic protein,MBP)免疫Lewis大鼠诱导EAE模型,采用随机数字表法分为模型组、激素组、中药组及正常对照组,分别在免疫后第7天、14天和28天采集血样,检测血浆细胞因子水平。结果免疫后第28天,模型组和激素组IL-4水平均显著下降,低于正常组(P<0.01),中药组IL-4水平升高,与正常组比较差异无统计学意义(P>0.05);免疫后第28天,模型组和激素组Th1/Th2水平均显著升高,与正常组比较差异有统计学意义(P<0.01),中药组Th1/Th2水平降低,与正常组比较差异无统计学意义(P>0.05)。结论二黄方可减轻EAE疾病严重程度,可能通过上调IL-4水平,下调Th1/Th2比值发挥对EAE的治疗作用。Objective To compare clinical course and the level of Th1/Th2 （]FN-γ/IL-4） from blood of lewis rats with experimental autoimmune encephalomyelitis （ EAE ） ; and to elucidate the functions of Erhuangfang in regulating autoimmune diseases. Methods Lewis rats were immunized with the myelin basic protein （MBP）. They were grouped randomly into normal group, EAE-group, prednisone group and Erhuangfang group post immunization（PI）. They were monitored daily after PI, and the clinical scores of the degree of paralysis were recorded. On day of 7, 14 and 28 PI, the peripheral blood were collected for cytokines examination. Results On day of 28 after PI, the level of IL-4 was significantly higher（ P 〈0.01 ） in Erhuangfang group than in EAE group and prednisone group. On day of 28 after PI, the Th1/Th2 level was significantly lower（P 〈 0.01 ） in Erhuangfang group than in EAE group and prednisone group. Conclusion Erhuangfang can alleviate the clinical signs, upregulate the IL--4 and downregulate the Th1/Th2 levels of serum in EAE.

关 键 词：自身免疫性疾病 实验性自身免疫性脑脊髓炎 细胞因子 中药 二黄方 

分 类 号：R285.5[医药卫生—中药学]