ATP对人鼻腔纤毛摆动频率的影响  

Effect of ATP on Human Nasal Ciliary Beat Frequency

作　　者：史凌改[1] 韩德民[1,2] 张罗[1,2] 

机构地区：[1]首都医科大学附属北京同仁医院耳鼻咽喉头颈外科 [2]北京市耳鼻咽喉科研究所 耳鼻咽喉头颈科学教育部重点实验室

出　　处：《首都医科大学学报》2009年第1期57-61,共5页Journal of Capital Medical University

基　　金：国家重点基础研究发展计划前期研究专项(2007CB516706);国家自然科学基金(30572026);教育部新世纪优秀人才支持计划(NCET-06-0185)资助项目~~

摘　　要：目的观察不同浓度三磷酸腺苷（adenosine triphosphate，ATP）对体外培养人鼻腔纤毛运动的影响。方法采用组织块培养法，应用高速数字化显微成像系统观察1umol／L、10umol／L和100umol／LATP对人鼻腔纤毛摆动频率（ciliary beat frequency，CBF）的影响。结果①Hanks平衡盐溶液（Hanks balanced salts solution，HBSS）对CBF无明显影响。②1umol／LATP使CBF由（9．2±1．8）Hz升至（10．3±2．0）Hz（n=10）（P〈0．05）。10umol／LATP使CBF由（9．5±1．7）Hz升至（11．5±2．0）Hz（n=10）（P〈0．05）。③100umol／LATP使CBF由（8．7±1．1）Hz升至（11．5±1．4）Hz（n=10）（P〈0．05）。结论ATP可刺激体外培养人鼻腔纤毛摆动频率升高，在1～100umol／L范围内，ATP对CBF的刺激呈浓度依赖性。Objective To investigate the effects of ATP on human nasal cilia movement. Methods Ciliary beat frequency（CBF） of cultured human nasal epithelial cells was measured by high-speed digital microscopy in ATP solution of different concentrations. Results CBF of cultured nasal epithelial cells exposed to HBSS showed no significant changes in 3 minutes （P 〉 0.05 ） ; However in 1, 10 and 100 umol/L ATP solution, CBF increased significantly in 10 seconds and reached the apex, then it showed a sustained elevation in 3 minutes. In 1 umol/L ATP solution, CBF increased significantly from （ 9.2 ± 1.8 ） Hz to （ 10.3 ±2.0 ） Hz （ n = 10 ） （ P 〈 0.05 ）. Similarly in 10 umol/L ATP and 100 umoL/L ATP solution, CBF increased significantly when compared with samples in HBSS. Conclusion When 1, 10 and 100umol/L ATP were added to the chamber, there were rapid increase in ciliary activity. In addition, there is concentration-dependent relation between CBF and ATP.

关 键 词：纤毛摆动频率 人鼻纤毛上皮细胞 三磷酸腺苷 

分 类 号：R765.25[医药卫生—耳鼻咽喉科]