^60Coγ线超大剂量照射人离体血早熟凝集染色体环的剂量-效应曲线  

A dose-effect curve of premature condensation chromosome ring in lymphocytes of human peripheral blood exposed to high dose of ^60Coγ-rays in vitro

作　　者：姚波[1] 李玉芳[1] 刘广贤[1] 黄珊[1] 蒋本荣[1] 艾辉胜[1] 

机构地区：[1]军事医学科学院附属医院全军放射病中心,北京100071

出　　处：《中华放射医学与防护杂志》2009年第1期54-56,共3页Chinese Journal of Radiological Medicine and Protection

摘　　要：目的建立超大剂量了线离体照射后人血淋巴细胞早熟凝集染色体环（PCC-R）的剂量一效应曲线。方法外周静脉血样采自3名健康男性，经^60Coγ线（剂量率2．35Gy／min）照射0、1、3、5、8、10、13、16、20、26和30Gy。于37℃恒温箱培养48h，收获前1h加入冈田酸以诱导产生早熟凝集染色体（PCC），计数PCC细胞中的PCC-R频率，拟合剂量．效应曲线。结果PCC指数随照射剂量增加而逐渐减少；每细胞PCC—R频率随照射剂量增加而增加直N20Gy，〉20Gy后趋于饱和。对所获数据进行回归分析，所拟合的曲线符合线性模型（上限剂量到20Gy）：Y=-0．020＋0．052D（γ为每细胞PCC-R频率，D为剂量，Gy）。结论本研究用药物诱导PCC—R法所建立的剂量-效应曲线可估计的上限剂量达20Gy，它的可靠性和实用性还有待于在今后的辐射事故中实际应用加以验证。Objective To establish a dose-effect curve of premature condensation chromosome ring （PCC-R）in lymphocytes of human peripheral blood after exposed to high doses of γ-rays. Methods Peripheral blood samples was drawn from three healthy individuals, and exposed to ^60Coγ-rays with doses between 0 and 30 Gy. The frequencies of PCC-R in premature condensation chromosome （PCC） cells obtained by Okadaic acid （OA） induction were calculated, and a dose-effect curve was fitted. Results PCC index tapered with dose. Frequencies of PCC-R per cell increased until 20 Gy, and then saturation was observed. The results were fitted to a lineal model up to 20 Gy： y = - 0.020 ＋ 0.052D ,where y was the frequencies of PCC-R per cell, D was the radiation dose（Gy）. Conclusions The highest dose could be estimated is 20 Gy by the dose-effect curve established with PCC-R method. Its utility and validity will be verified in the future application of radiation accident.

关 键 词：大剂量照射 早熟凝集染色体环 剂量-效应曲线 

分 类 号：R144.1[医药卫生—公共卫生与预防医学]