β-半乳糖苷酶在ROSAβgeo26小鼠衍生的神经干细胞中的表达变化  

Expression of β-galactosidase in the neural stem cells of ROSAβgeo26 mouse

作　　者：方杰[1] 苏阳[1] 李秀苗[1] 肖明[1] 丁炯[1] 韩群颖[1] 

机构地区：[1]南京医科大学人体解剖学系,江苏南京210029

出　　处：《解剖学研究》2009年第1期14-16,53,共4页Anatomy Research

基　　金：江苏省自然科学基金(BK2006576;BK2006232)

摘　　要：目的观察β-半乳糖苷酶(β-gal)在ROSAβgeo26小鼠衍生的神经干细胞(NSCs)中的表达变化。方法体外培养NSCs,并应用X-Gal组织化学和免疫细胞化学染色技术研究NSCs中β-gal的表达变化。结果原代克隆球和亚克隆球的NSCs均稳定表达β-gal;在NSCs分化过程中,NeuN阳性的神经元、GalC阳性的少突胶质细胞和GFAP阳性的星形胶质细胞均表达β-gal,但表达水平有所下降。结论ROSAβgeo26小鼠衍生的NSCs能稳定的表达β-gal,可用作NSCs研究的细胞来源。但在干细胞分化的相关研究中,值得注意存在β-gal不同程度的表达下调现象,并应进一步关注出现该现象的潜在机制。Objective To observe the expression of β-galactosidase （β-gal） in the neural stem cells （NSCs） of ROSAβgeo26 mouse. Methods NSCs were cultured in vitro. Then X-Gal histochemistry and immunocytochemistry were performed to study the expression of β-gal in the NSCs. Results β-gal was stably expressed in the primary clones and subclones of NSCs. During the differentiation of NSCs, β-gal activity was colocalized to NeuN positive neurons, GalC positive oligodendrocytes and GFAP positive astrocytes, but the level of β-gal expression was down-regulated. Conclusion β-gal is stably expressed in the NSCs of ROSAβgeo26 mouse. This strain of mice may serve as a promising resource for study of NSCs. But in the research related to NSCs differentiation, β-gal down-regulated expression of raring degrees and its underlying mechanism should be noted.

关 键 词：Β-半乳糖苷酶 X—Gal 转基因小鼠 神经干细胞 

分 类 号：R341[医药卫生—基础医学] R329