新维甲类化合物联合γ干扰素对U937细胞增殖分化作用及其机制的研究  

Study on effects of new retinoids in combination with IFNγ on proliferation and differentiation of U937 cell and its mechanism

作　　者：陈惠仁[1,2,3,4,5] 孙关林[1,2,3,4,5] 李晶[1,2,3,4,5] 韩锐 何小庆[1,2,3,4,5] 苏卉[1,2,3,4,5] 邬维礼 钱六妹[1,2,3,4,5] 王振义 

机构地区：[1]上海第二医科大学 [2]上海血液学研究所 [3]北京空军总医院 [4]镇江医学院附院 [5]中国医学科学院中国协和医科大学药物研究所

出　　处：《中华血液学杂志》1998年第2期70-73,共4页Chinese Journal of Hematology

基　　金：国家九五攻关课题基金;胡应洲奖励基金部分资助

摘　　要：目的：了解新维甲类化合物联合γ干扰素（ＩＦＮ－γ）对Ｕ９３７细胞的增殖分化作用及其机制。方法：检测Ｕ９３７细胞ＮＢＴ阳性率、细胞表面ＣＤ１４和ＣＤ６８表达、ｃ－ｍｙｃ和细胞周期变化，研究新维甲类化合物Ｒ９１５８和ＹＳ９０４０１２分别联合ＩＦＮ－γ对Ｕ９３７细胞增殖分化的作用。结果：先用维甲类化合物作用２４小时后换用ＩＦＮ－γ诱导Ｕ９３７细胞分化，有明显的协同作用；若用相反顺序处理，它们的协同作用明显降低。新维甲类化合物２４小时内能使细胞积聚于Ｇ０／Ｇ１期，下调ｃ－ｍｙｃ基因。ＹＳ９０４０１２活性稍强。结论：新维甲类联合ＩＦＮ－γ有协同诱导Ｕ９３７细胞分化的作用。维甲类化合物主要作用于细胞的早期阶段；ＩＦＮ－γ主要在后期起作用。早期应用维甲类化合物在促进细胞分化中具有重要的作用。Objective:To illustrate the effects of new retinoids in combination with IFNγ on the proliferation and differentiation of U937 cell and its mechanism.Method:Effects of new retinoids R9158,YS904012 in combination with IFNγ on the proliferation and differentiation of U937 cells were studied by detecting NBT,expression of CD14, CD68 and cmyc,and cell cycle shift.Result:When the U937 cells had been first treated with new retinoid for 24 hours,and then with IFNγ,the differentiation activity could be enhanced,the reverse of the use of two agent in chronology displayed much lower synergistical effect.Retinoids could down regulate cmyc expression and shift the cell population from S phase to G0/G1 phase within 24 hours, and the activity of YS904012 was greater than that of other retinoids.Conclusion:New retinoids in combination with IFNγ synergistically induced U937 differentiation.Retinoids may play an important role in triggering the differentiation.

关 键 词：维生素A Γ干扰素 U937细胞 增殖分化 

分 类 号：R977.21[医药卫生—药品]