血清透明质酸时间分辨荧光免疫分析法的反应条件优化及其临床应用  

Optimization of reaction conditions of the time-resolved fluoroimmunoassay for hyaluronic acid detection in serum and its clinical application

作　　者：朱岚[1] 黄飚[1] 张珏[1] 裴豪[2] 刘海燕[1] 

机构地区：[1]江苏省原子医学研究所,江苏无锡214063 [2]无锡市传染病医院检验科,江苏无锡214065

出　　处：《检验医学》2009年第2期124-126,共3页Laboratory Medicine

基　　金：江苏省社会发展基金资助项目(BS2004009)

摘　　要：目的优化血清透明质酸（HA）时间分辨荧光免疫分析法（TRFIA）的反应条件,并探讨其临床应用价值。方法采用TRFIA检测血清中HA的含量,确定参与HA-TRFIA的各反应成分的浓度。结果最佳反应条件为包被浓度10 mg/L、HA结合蛋白（HABP）1∶500稀释、铕标记HA（Eu^3＋-BSA-HA）1∶200稀释。敏感性为5.17μg/L,批内变异系数（CV）为2.21%-3.78%,批间CV为3.73%-5.40%,平均回收率为100.4%。该法HA测定值与放射免疫测定值显著相关,且与临床结果一致。结论本研究建立的血清HA-TRFIA是一种灵敏准确的分析方法,适合临床应用。Objective To optimize the reaction conditions of the time-resolved fluoroimmunoassay（TRFIA）for hyaluronic acid （HA） detection in serum and explore its clinical application. Methods The serum levels of HA were detected by TRFIA. The different concentrations of the compositions in the HA-TRFIA system were set up. Results The optimum concentrations of goat anti-rabbit IgG, HA binding protein（HABP） and europium-labeled HA （Eu^3＋ -BSA-HA） were 10 mg/L,1： 500 and 1： 200, respectively. The analytical sensitivity was 5. 17 ug/L. The within- run and be- tween-run coefficients of variation were 2.21% -3.78% and 3.73% -5.40% , respectively. The average recovery rate was 100.4%. The results had significant co-relationship with radioimmunoassay, and was consistent with the clinical diagnosis. Conclusions The competitive TRFIA for HA detection in serum is a sensitive and reliable assay. It would be suitable for clinical diagnosis.

关 键 词：时间分辨荧光免疫分析法 反应条件 优化 透明质酸 

分 类 号：R446.61[医药卫生—诊断学]