薄层扫描法测定熟地黄中果糖的含量  

TLC Scanning for the Determination of Fructose in Radix Rehmanniae Preparata

作　　者：李军[1] 张丽萍[1] 游志恒[1] 王晓阁[1] 

机构地区：[1]河南中医学院,河南郑州450008

出　　处：《河南中医学院学报》2009年第1期56-58,共3页Journal of Henan University of Chinese Medicine

基　　金：国家科技部"十五"科技攻关重大科研项目(编号:2001BA701A551-22)

摘　　要：目的:采用双波长薄层扫描法对熟地黄中的果糖进行定量。方法:薄层板为硅胶G板,以正丁醇-吡啶-水(16∶15∶4)为展开剂,以380 nm为测定波长,465 nm为参比波长。结果:熟地黄果糖在1.88～9.40μg之间呈良好线性关系,平均回收率为99.58%,RSD为2.713%(n=6)。结论:采用本方法测定熟地黄中果糖的含量是可行的。Objective： To determine fructose in Radix rehmanniae preparata by TLCS. Methods： TLC was Silica gel G, n - Butanol - Pyridine-Water （16：15：4） was used as development, sample wave was 380 nm, reference wave was 465 nm. Results： The linear range was form 1.88 to 9.40ug （r= 0. 999）. The average recovery rate was 99.58%, RSD was 2. 713% （ n = 5）. Conclusion： The methods can control the quality of this preparata effectively.

关 键 词：薄层扫描 熟地黄 果糖 中药化学分析 

分 类 号：R284.1[医药卫生—中药学]