O型口蹄疫病毒各编码基因及其产物变异率的分析  

The Analysis of the Mutation Rate of Encoding Sequences and Their Products in Serotype O Foot-and-Mouth Disease Virus

作　　者：周建华[1] 丛国正[1] 常惠芸[1] 

机构地区：[1]中国农业科学院兰州兽医研究所,家畜疫病病原生物学国家重点实验室,农业部畜禽病毒学重点开放实验室,国家口蹄疫参考实验室,甘肃兰州730046

出　　处：《华北农学报》2009年第1期60-63,共4页Acta Agriculturae Boreali-Sinica

基　　金：国家科技支撑计划(2006BAD06A14;2006BAD06A10)

摘　　要：将从Genbank中提出的20株O型FMDV的全基因组分割成12个编码区域,即Lab、VP4、VP2、VP3、VP1、2A、2B、2C、3A、3B、3C和3D。通过方差分析,12个编码序列的相对变异率之间无差异(P>0.05);而这12种编码序列所对应的氨基酸相对突变率之间差异显著(P<0.01)。利用Duncan法对这12种氨基酸序列相对突变率进行多重比较,发现3A蛋白的氨基酸相对突变率比其余11种蛋白的差异都显著(P<0.05);VP2、Lpro的氨基酸相对突变率与VP4、2A、2C、3Cpro、3Dpol之间存在差异(P<0.05);而VP4、2A、2C、3Cpro、3Dpol之间无差异。结果显示,O型FMDV自身RNA的转录和机体对FMDV施加的免疫压力不是造成FMDV变异的主要原因,而各种病毒产物的功能选择压力才是FMDV分子进化的主要原因。A total of 20 foot-and-mouth disease virus （FMDV）serotype O was obtained from Genbank and was divided into 12 encoding sequences（Lab, VP4, VP2, VP3, VP1,2A ,2B,2C,3A ,3B,3C and 3D） .After analyzing the difference among them by analysis of variance, at the nucleotide level, the relative mutation rates of these 12 encoding sequences were no difference （ P 〉 0.05）, however, at the amino acid level, those relative mutation rates were different（ P 〈 0.01 ）. And the analysis of Duncan method indicated that the relative mutation rate of 3A protein is the highest. Comparison with VP4,3C^pro ,3D^pol,2A,2C, the relative mutation rates of VP2 and L^pro were different than them（ P 〈 0.05）. In addition, the relative mutation rates of VP4,3C^pro, 3D^pol,2A, 2C were no different. The proposed results strongly suggested that serotype O FMDV RNA transcription and the pressure of immunity failed to play an important role in driving FMDV genome evolution. An evolutionary pressure is related to functional conservation.

关 键 词：口蹄疫病毒 相对变异率 方差分析 Duncan法 

分 类 号：Q785[生物学—分子生物学]