TAIL-PCR技术分离柞蚕丝素基因3′端非编码区未知DNA序列  

TAIL-PCR System for Isolation of an Unknown DNA Sequence Lying Inside the 3′Noncoding Region of the Fibroin Gene

作　　者：冷春玲[1] 

机构地区：[1]辽东学院农学院生物系,辽宁丹东118003

出　　处：《华北农学报》2009年第1期108-111,共4页Acta Agriculturae Boreali-Sinica

摘　　要：以柞蚕基因组DNA为材料,采用热不对称交错PCR(TAIL-PCR)技术分离出1 998 bp的DNA序列。将该序列与Genebank上的柞蚕丝素基因DNA序列(AF083334)进行比较分析结果表明,新钓取的DNA片断中1 758 bp的DNA序列为柞蚕丝素基因3′端非编码区未知DNA序列。TAIL-PCR技术为柞蚕丝素基因3′端非编码区未知DNA序列的钓取提供了一种简便、高效的方法。1 998 bp DNA sequence was isolated here with genomic DNA of Antheraea pernyi by Thermal Asymmetric Interlaced PCR（TAIL-PCR）. The results from comparing 1 998 bp DNA sequence with the sequence that already landed on the Genebank（AF083334）showed that the DNA sequence（1 758 bp）in the newly isolated DNA segments was an unknown DNA sequence lying inside the 3＇ noncoding region of the fibroin gene. The method we describe here provides a simple, rapid and efficient means to isolate an unknown DNA sequence lying inside the 3＇ noncoding region of the fibroin gene.

关 键 词：TAIL-PCR 丝素基因 简并引物 侧翼序列 

分 类 号：Q78[生物学—分子生物学]