西瓜强雌性性状的遗传分析及分子标记研究  

Inheritance Analysis and Molecular Marker of the Subgynoecious Trait in Watermelon

作　　者：张秦英[1] 刘军伟[1] 刘莉[1] 焦定量[2] 郭富常[1] 郭敏[1] 

机构地区：[1]天津大学农业与生物工程学院,天津300072 [2]天津科润蔬菜研究所,天津300384

出　　处：《华北农学报》2009年第1期138-142,共5页Acta Agriculturae Boreali-Sinica

基　　金：天津市应用基础研究计划项目(07JCZDJC04200)

摘　　要：为了解西瓜强雌性性状的遗传规律,以强雌性系BG1为母本,分别与不同的西瓜品系杂交、自交及回交,共获得5个F1群体、1个F2群体、1个回交群体BC1R34,以及1个回交转育品系(BC4S3)。5个F1群体植株全部表现为正常花性型;经χ2测验F2分离群体中强雌性型与普通花性型的分离符合1∶3的分离比率;与普通花性型回交后代的BC1R34表现为普通花性型;回交转育得到的BC4S3品系表现为强雌性型,雌花率高达77.9%。试验结果表明,该强雌性状主要受单隐性核基因控制,但根据F2单株雌花率的分布情况,认为还可能存在微效基因的作用。对两对近等基因系BG1和BG2及BC4S3和R34进行RAPD分析,共使用333条随机引物,经过进一步F2验证在强雌性系中获得一条稳定扩增的片段s1454-1100。The objective of this experiment was to investigate the inheritance of subgynoecious trait in watermelon. Five F1 combinations, one F2 segregation population, one backeross population（ BC1R34）and one transferred subgynoecious line（ BC4S3）were developed by crossing, selfing and backcross between subgynoecious line（ BG1 ）and other watermelon inbred lines, respectively. All the plants of F1 populations expressed monoecious, similar to their male parents ; F2 population segregated into two phenotypes of normal monoecious type and subgynoecious type in a ratio of 1 ： 3 through X^2 test ; as well the plants of backcross population BC1 R34 completely expressed monoecious. In addition, transferred subgynoeeious line BC4S3 was subgynoecious type that produced high female flower proportion to 77.9 %. These results indicated that subgynoecious in watermelon was controlled by a single recessive gene. According to the frequence distribution of female flower proportion in F2, it was considered that there might be other minor genes modified this subgynoecious trait. Two pairs of NILs, BG1 versus BG2, and BC4S3 versus R34 was studied by the RAPD technique out of 333 random primers. A specific amplified fragment s1454-1100 in subgynoecious line was found out through verifying the F2 individuals.

关 键 词：西瓜 强雌性系 遗传分析 RAPD 

分 类 号：S651[农业科学—果树学]