牛早期胚胎性别鉴定快速PCR方法的研究  

Sex Identification of Bovine Embryo with PCR Methods

作　　者：张莉[1] 史爱华[1] 李桂萍[1] 余文莉[1] 

机构地区：[1]北京市农林科学院畜牧兽医研究所,北京100097

出　　处：《华北农学报》2009年第1期212-214,共3页Acta Agriculturae Boreali-Sinica

基　　金：北京市优秀人才资助项目

摘　　要：根据公牛Y-染色体特异重复序列和公母牛共有的DNA序列，设计并合成PCR引物用于牛或牛胚胎性别鉴定，通过PCR反应对公母牛静脉血及牛胚胎进行性别鉴定，结果表明，母牛只能扩增出250bp的内标基因片段，而公牛能同时扩增出131bp的Y-染色体特异性基因片段和250bp的内标基因片段。静脉血样与实际性别的符合率为100％；4～10个胚胎细胞的扩增也出现和静脉血相同的特异条带。这表明该检测方法准确、简便，具有较高的灵敏性和稳定性。Two pairs of specific primes were designed on the special sequences of Y chromosome and sharing DNA sequence of bull and cow, a set of polymerize china reaction were used for identify the bull and cow blood samples and embryo. Results showed cow only demonstrated a band of 250 bp and bull demonstrated two band of 131 bp by and 250 bp,resuhs of blood identification is completely in accordance with the actual sex type,4 to 10 embryo cell also demonstrated same specific band model of blood sample, which showed that this method of sex identification were effective and sensitive.

关 键 词：牛胚胎 性别鉴定 PCR 

分 类 号：S823[农业科学—畜牧学]