全苯异氰酸酯化环糊精固定相对手性药物的拆分  

Separation of Five Medicines on a New β-cyclodextrin Derivative Bonded Chiral Stationary Phase

作　　者：周婕[1] 王艳芝[1] 张振中[1] 

机构地区：[1]郑州大学药学院药物分析系,郑州450001

出　　处：《应用化学》2009年第3期355-357,共3页Chinese Journal of Applied Chemistry

基　　金：教育部高等学校博士学科点专项科研基金(20040335045)资助项目

摘　　要：采用分步法制备了全苯异氰酸酯化β-环糊精键合硅胶手性固定相,并采用高效液相色谱分析技术对外消旋氟西汀、帕罗西汀、萘普生、普萘洛尔和阿普洛尔进行了拆分。实验结果发现,5种手性药物在自制的手性固定相上均获得基线分离,并且得到了最佳的色谱条件:帕罗西汀、萘普生为甲醇-0.15%TEAA(体积比35∶65,pH=4.0);氟西汀为甲醇-0.15%TEAA(体积比30∶70,pH=4.0);普萘洛尔、阿普洛尔为甲醇-0.15%TEAA(体积比40∶60,pH=5.0)。Several chiral medicines were separated on a new home-made β-cyclodextrins chiral stationary phase. The perphenylcarbamoylated β-cyclodextrin bonded silica CSP was prepared step by step, from synthesized and purified mono-6-p-toluenesulfonyl-β-eyclodextrin, mono-6-N-allylamino-β-cyclodextrin and mono-6-N-allylamino-perphenylcarbamoylated-β-eyclodextrin. By this CSP, baseline separation of five racemie medicines （ fluoxetine, paroxetine, naproxen, propranolol and alprenolol） was realized. The optimal conditions were determined. The mobile phase was methanol-0. 15% TEAA （ 35： 65, V/V, pH = 4, 0） for paroxetine and naproxen, methanol-0. 15% TEAA （ 30 ： 70, WV, pH = 4. 0 ） for fluoxetine, and methanol-0. 15% TEAA （40：60, V/V, pH = 5.0 ） for propranolol and alprenolol, respectively; the flowrate was 0. 5 mL/min, the temperature 20℃, and UV-deteetor was used（254 nm）.

关 键 词：环糊精衍生物 手性固定相 药物对映体 拆分 高效液相色谱 

分 类 号：O652[理学—分析化学]