烟草根特异性启动子植物表达载体的构建及其对番茄的转化  

Construction of expression vector driven by tobacco root-specific promoter and Agrobacterium-mediated transformation of tomato

作　　者：彭娟[1,2] 李志邈[1] 杨悦俭[1] 叶青静[1] 吴才君[2] 张小明[3] 

机构地区：[1]浙江省农业科学院蔬菜研究所,浙江杭州310021 [2]江西农业大学农学院,江西南昌330045 [3]浙江省农业科学院作物与核技术利用研究所,浙江杭州310021

出　　处：《浙江农业学报》2009年第1期1-5,共5页Acta Agriculturae Zhejiangensis

基　　金：浙江省自然科学基金项目(Y305280)

摘　　要：利用PCR技术从烟草中克隆获得了893bp的根特异性表达基因TobRB7的启动子,命名为NT1。以pBI121为原始植物表达载体,用NT1启动子和番茄广谱抗病基因Pto分别取代CaMV 35S启动子和GUS基因,获得了烟草根特异启动子驱动抗病基因的表达载体,命名为pBI-NT1::Pto。采用冻融法将pBI-NT1::Pto导入根癌农杆菌EHA105中,以番茄子叶为外植体进行了侵染转化。PCR检测NPT Ⅱ基因的结果表明已获得转基因番茄植株。The 893bp fragment of root-specific promoter of tobacco TobRB7 gene, namely NT1, was cloned by PCR. The expression vector pBI-NT1 ： ： Pro, driven by tobacco root-specific promoter NT1, was constructed from pBI121 by substituting the CaMV 35S promoter and GUTS gene with NT1 and Pro, a tomato broad-spectrum R gene, respectively. Through freeze and thaw method, the pBI-NT1 ： ： Pro vector was introduced into Agrobacterium tumefaciens strain EHA105, and the explants of tomato cotyledons were then transformed. PCR test result of NPT H gene indicated that transgenic tomato lines were obtained.

关 键 词：番茄 根特异启动子 表达载体 转基因 

分 类 号：Q344[生物学—遗传学]