盐附子遗传毒性研究  

GENOTOXIC RESEARCH ON SALTED EXTRACT OF CRUDE LATERAL ROOT OF ACONITE

作　　者：陈红钰[1,2] 王亚其[1] 潘黎[1,2] 卢豪[1] 严光焰[1,2] 李宏霞[1] 

机构地区：[1]四川大学华西医院,国家(成都)中药安全性评价中心,成都610041 [2]四川大学华西公共卫生学院

出　　处：《现代预防医学》2009年第5期917-919,共3页Modern Preventive Medicine

基　　金：国家自然科学基金资助项目(30230410)

摘　　要：[目的]选用目前新药遗传毒性评价中推荐使用的3种试验方法(小鼠骨髓微核试验、鼠伤寒沙门氏菌回复突变试验、体外CHL细胞染色体畸变试验)研究盐附子的遗传毒性。[方法]小鼠骨髓微核试验设3个盐附子给药剂量组(15.45、7.73、3.86g生药/kg)、阴性对照组和阳性对照组(环磷酰胺40mg/kg)。Ames试验选用两种方法:直接法和代谢活化法。试验菌株为鼠伤寒沙门氏组氨酸缺陷型菌株TA97、TA98、TA100、TA102、TA1535,盐附子在试验中设6个剂量组(5.000、2.500、1.250、0.625、0.313、0.156mg生药/皿),同时设自发回复突变对照组、阳性对照组。体外CHL细胞染色体畸变试验也选用两种方法:直接法和代谢活化法。盐附子不加S9时处理终浓度为5、2.5、1.25、0.625、0.313mg生药/ml,加S9时处理终浓度为2.5、1.25、0.625、0.313、0.156mg生药/ml。[结果]小鼠骨髓微核试验盐附子各剂量组小鼠骨髓多染红细胞微核率与阴性对照组相比,差异无统计学意义(P﹥0.05)。Ames试验盐附子在加或不加肝微粒体酶(S9)时均未见引起TA97、TA98、TA100、TA102、TA1535试验菌株基因突变,即Ames试验阴性,试验重复一次,所得结论相同。体外CHL细胞染色体畸变试验盐附子在加或不加S9时均未引起CHL细胞的染色体畸变,试验结果为阴性,试验重复一次,所得结果相同。[结论]在实验室条件下,盐附子在小鼠骨髓微核试验、Ames试验及体外CHL细胞染色体畸变试验中均为阴性结果,故认为盐附子无遗传毒性。[Objective] The salted extract of crude lateral root of aconite was evaluated on genotoxcity in this study. [Methods] The Micronuclei assay in mice, Salmonella reverse mutation assay and Chromosome aberration assay were applied in the study. [Results] There was no significant difference in micronuclei frequency of mice bone marrow cell between all dosing groups of the treatment groups and negative control （P﹥0.05）.The number of revertant colony of dosing groups did not reach to two times of the number corresponding of spontaneous revertant colony. The frequency of chromosome aberration in dosing groups of the extract was no significant difference to negative control （P﹥0.05） at 24h and 48h respectively. [Conclusion] The results imply that the salted extract of crude lateral root of aconite has no genotoxicity.

关 键 词：盐附子 遗传毒性 小鼠骨髓微核试验 AMES试验 染色体畸变试验 

分 类 号：R28[医药卫生—中药学]