猪干扰素-γ基因的克隆及其真核表达载体的构建  

Clone of porcine interferon-γ gene and construction of its eukaryotic expression plasmids

作　　者：侯小康 韩柱 

机构地区：[1]河北中润制药有限公司,河北石家庄050046 [2]华北制药新药研究开发有限公司,河北石家庄050015

出　　处：《齐鲁药事》2009年第2期110-113,共4页qilu pharmaceutical affairs

摘　　要：目的克隆猪的干扰素-γ基因(PoIFN-γ),构建重组真核表达质粒,转染细胞。方法从猪的肝脏组织中提取出基因组DNA,以其为模板通过长链PCR扩增出干扰素-γ全基因。将猪干扰素γ基因克隆到真核表达载体PCI-neo载体中,组装成真核表达载体PCI-neo-PoIFNγ。利用脂质体转染法将重组载体PCI-neo-PoIFN-γ转染入中国仓鼠卵巢细胞(CHO)中,在G418的存在下进行筛选培养。结果构建出重组真核表达质粒,获得转染细胞系。结论成功克隆了猪的干扰素-γ基因,获得了转染细胞系。OBJECTIVE Cloning Porcine interferon-γ gene, re-construction of eukaryotic expression plasmid, and transfected cells. METHODS From Porcine liver tissue,genomic DNA was extracted and the interferon-γ gene was cloned by PCR amplification. Porcine interferon-γ, gene was cloned into the eukaryotic expression vector PCI-neo vector and assembled into a eukaryotic expression vector PCI-neo-PoIFN-γ. The recombinant vector PCI-neo-PoIFN-γ wased transfer into Chinese hamster ovary cells （CHO） using lipofectamine the strain was screened, and screening training in the presence of G418. RESULTS A eukaryotic expression vector was constructed and transfected cell lines. CONCLUSION We have successfully cloned a Porcine interferon-γ gene and transfected cell lines.

关 键 词：猪干扰素-γ 真核表达载体 中国仓鼠卵巢细胞 

分 类 号：R978.7[医药卫生—药品]