猪轮状病毒性腹泻的ELISA双抗夹心法检测及防治建议  

Detection on Porcine Rotavirus by ELISA Double Sandwich Method and Its Prevention Suggestion

作　　者：王玲[1] 蒲万霞[1] 孟小琴[2] 邓海平[3] 崔东安[1] 焦硕[1] 杨志强[1] 

机构地区：[1]中国农业科学院兰州畜牧与兽医研究所新兽药工程重点开放实验室,甘肃兰州730046 [2]甘肃农业大学动物医学院,甘肃兰州730070 [3]西北师范大学生命科学学院,甘肃兰州730070

出　　处：《安徽农业科学》2009年第5期1990-1990,2013,共2页Journal of Anhui Agricultural Sciences

基　　金：甘肃省自然科学基金项目(0710RJZA080);农业部畜禽病毒学重点开放实验室资助课题(200601-200712)

摘　　要：[目的]为猪轮状病毒的针对性防治提供建议。[方法]以不同来源、不同饲养方式下218头猪的粪便为材料,用ELISA双抗夹心法检测其中的轮状病毒(RV)抗原,结合流行病学调查对猪轮状病毒的发病因素及发病特点进行分析。[结果]RV多为隐性感染,且感染率较高。从所取218份样品中共检测出RV阳性50头份,阳性率为22.94%,其中,仔猪170头份,阳性率22.35%,中猪48头份,阳性率25.0%;笼养模式下猪的发病率最低,为17.43%,农户圈养方式下猪的发病率最高,达36.84%。[结论]流行病学调查结果显示,仔猪对RV的感染率高于中猪,采取笼养模式,并对仔猪进行RV防治可降低猪RV的发病率。[Objective] The study was to provide the suggestions for the appropriate prevention on porcine rotavirus. [Method] With stool of 218 pigs from different sources and feeding manners as the materials, the antigen of porcine rotaviras （RV） in them was detected by sandwich ELISA. Combined with epidemiology survey, the pathogenic factors and characters of porcine rotavirus were analyzed. [Result] Most RV were inapparent infection and its infection rate was higher. Total 50 pigs with positive RV were detected from 218 pigs and the positive rate was 22.94%. Among which, there were 170 piglets and their positive rate was 22.35%, while the medium pig was 48 and its positive rate was 25.0%. The pig incidence in captivity was the lowest, being 17.43%, while that in captive was the highest, being 36.84%. [Conclusion] The investigation results of epidemiology indicated that the RV infection rate of piglets was higher than that of medium pig. Taking captivity and prevention RV from piglets could decrease the V infection rate of pig.

关 键 词：猪轮状病毒 流行病学 ELISA双抗夹心法 

分 类 号：S858.28[农业科学—临床兽医学]