恩诺沙星纳米粒的制备及稳定性研究  

Study on the Preparation and the Stability of Enrofloxacin Nanoparticles(ENR-NPs)

作　　者：龚露旸[1] 杨先乐[1] 胡鲲[1] 

机构地区：[1]上海海洋大学国家水生动植物病原库,上海201306

出　　处：《安徽农业科学》2009年第5期2019-2021,共3页Journal of Anhui Agricultural Sciences

基　　金：国家"十一五"科技支撑计划项目(2006BAD03B04)

摘　　要：[目的]优化恩诺沙星纳米粒制备参数,考察其稳定性。[方法]以壳聚糖为辅料,采用离子交联法和冷冻干燥法制备恩诺沙星壳聚糖纳米粒,以包封率为参考指标设计正交试验,确定优化制备参数,以透射电镜观察其表观特征,并对其热稳定性及紫外稳定性进行考察。[结果]以优化参数制备的恩诺沙星壳聚糖纳米粒平均粒径237.4 nm,平均包封率(71.9±2.2)%,平均载药量(12.4±0.6)%。与原料药相比,4 h热降解率和紫外降解率分别减少了38.4%和13.3%。[结论]离子交联法和冷冻干燥法适用于制备恩诺沙星壳聚糖纳米粒,其产品具有良好的热稳定性和紫外稳定性。[Objective] The research aimed to optimize the preparing method of enrofloxacin nanoparticles （ENR-NPs） and investigate its stability. [Method] ENR-NPs was prepared using freeze-drying and ionic gelation method with chitosan. The thermal and ultraviolet stability were measured at the same time. [Result] The nanoparticlas observed by TEM were spherical with a mean particle size of 237.4 nm. The encapsulation efficiency and drug loading of ENR-NPs were （71.9 ± 2.2）% and （12.4 ± 0.6）%. The total thermal and ultraviolet degradation of ENR-NPs in 4 h were decreased by 38.4% and 13.3% compared to raw ENR. [Conclusion] Ionic gelation and freeze-drying method ia applicable to the preparation of ENR-NPs and its product has characteristic of good thermal and ultraviolet stability.

关 键 词：恩诺沙星 壳聚糖 纳米粒 热稳定性 紫外稳定性 

分 类 号：S859[农业科学—临床兽医学]