人单核细胞趋化蛋白-15'端翻译起始区的改构及其表达  

OPTIMIZATION AND EXPRESSION OF MCP1 5TRANSLATION INITIATION REGION

作　　者：张毅[1,2] 叶棋浓[1,2] 王恒梁[1,2] 刘及[1,2] 苏国富[1,2] 

机构地区：[1]军事医学科学院生物工程研究所 [2]白求恩医科大学

出　　处：《中国免疫学杂志》1998年第1期18-21,共4页Chinese Journal of Immunology

摘　　要：应用ＰＣＲ技术优化人单核细胞趋化蛋白－１（ＭＣＰ－１）基因的５端翻译起始区，并将其亚克隆入大肠杆菌表达载体ｐＢＶ２２０中，经ＤＮＡ测序证实优化成功，且在大肠杆菌中表达出ＭＣＰ－１蛋白，表达产物约占菌体总蛋白的１０％左右，Ｗｅｓｔｅｒｎｂｌｏｔ检测表明，表达产物可与ＭＣＰ－１抗体发生特异性反应。he 5translation initiation region of human monocyte chemoattractant protein1(MCP1) was optimized by PCR and the optimized MCP1 gene was cloned into pBV220. DNA sequence indicated that it was correct. MCP1 was expressed successfully at the level of about 10% of total proteins in E.coli. Western blot analysis showed that the expressed product was reacted specifically with antiMCP1 antibodies.

关 键 词：人单核细胞趋化蛋白-1 PCR 原核表达 

分 类 号：R392.12[医药卫生—免疫学]