侧脑室注射孤啡肽对创伤大鼠NK细胞活性的影响  

EFFECT OF INTRACEREBROVENTRICULAR INJECTION OF ORPHANIN FQ ON NK CELL ACTIVITY OF TRAUMATIC RATS

作　　者：杜莅娜[1] 姜建伟[1] 吴根诚[1] 曹小定[1] 

机构地区：[1]上海医科大学医学神经生物学国家重点实验室

出　　处：《中国免疫学杂志》1998年第1期33-36,共4页Chinese Journal of Immunology

基　　金：卫生部;国家中医药管理局及国家攀登计划

摘　　要：采用侧脑室注射（ｉｃｖ）孤啡肽（ＯｒｐｈａｎｉｎＦＱ，简称ＯＦＱ）和反义核苷酸（ＡＳＯ）的技术，观察了ＯＦＱ对手术创伤应激介导的自然杀伤细胞（ＮＫ细胞）活性下降效应的影响，结果表明：正常大鼠ｉｃｖＯＦＱ０．１μｇ（０．０５５ｎｍｏｌ）和１．０μｇ（０．５５０ｎｍｏｌ）ＮＫ细胞活性与生理盐水组相比无明显差异。５．０μｇ（２．７５０ｎｍｏｌ）明显抑制正常ＮＫ细胞活性（Ｐ＜０．０５），手术创伤后大鼠的ＮＫ细胞活性明显低于正常对照组（Ｐ＜０．００１）。０．１μｇ、１．０μｇ和５．０μｇ的孤啡肽（ｉｃｖ）均能明显地拮抗由创伤导致的ＮＫ细胞活性抑制（Ｐ＜０．０５），采用ＡＳＯ技术阻断ＯＦＱ的合成后，再给予ＯＦＱ（１．０μｇ，ｉｃｖ）对创伤大鼠的免疫低下效应无影响。研究提示脑内ＯＦＱ在调整创伤应激介导的免疫低下效应中起重要的作用。he present paper investigated the influence of intracerebroventricular(icv) injection of orphanin FQ(OFQ)(a newly discovered 17amino acid peptide) and ASO(an antisense oligonucleotide to OFQR) on the NK cell activity from the traumatic rats. All the injected volumes were 20 μl. The NK cell activity was determined by europiumlabelled target cell based on timeresolved fluorometryCompared to the normal saline group, NK cell activity have no change in icv OFQ 10 μg(0.550 nmol) and 0.1 μg(0.055 nmol) groups, icv injection of OFQ 50 μg(2.750 nmol) led to a suppression of the splenic NK cell activity. 50 μg,10 μg and 0.1 μg icv OFQ were all displayed to significantly antagonised the immune depression produced by surgical trauma. When OFQ was given after the ASO treatment, the OFQ induced antiimmunosuppression effect was completely reversed. These results suggest that OFQ takes part in the regulation of immunosuppression produced by surgical trauma.

关 键 词：孤啡肽 创伤 自然杀伤细胞 反义核苷酸 

分 类 号：R641.03[医药卫生—外科学]