应用PCR技术检测血清抗双链DNA抗体  

DETECTION OF ANTIDOUBLESTRANDED DNA ANTIBODIES BY PCR METHOD

作　　者：俞乃昌[1] 徐陆亭[1] 周源 杨丽萍[1] 

机构地区：[1]北京宣武医院临床免疫科

出　　处：《中国免疫学杂志》1998年第1期43-45,共3页Chinese Journal of Immunology

基　　金：北京市自然科学基金

摘　　要：人工合成一段６２核苷酸的核苷酸序列，中间２５个核苷酸为随机合成。经ＰＣＲ扩增后提供一个含有亿万种结构变异ＤＮＡ片段库供抗ＤＮＡ抗体反应。阳性病人血清沉淀的特异双链ＤＮＡ片段经ＰＣＲ扩增后出现特异的阳性扩增区带。结果表明４０例ＳＬＥ血清经ＰＣＲ检测３２例为阳性（８０％），而Ｆａｒｒ放免法１４例为阳性（３５％），两者之间有显著差异（Ｐ＜０．００５），４０例非ＳＬＥ结缔组织病人血清，两者阳性率分别为７．５％和２．５％，两者比较无明显差异（Ｐ＞０．０２５）。４０例健康献血员血清的ＰＣＲ与放免结果均为阴性。ＰＣＲ方法能有效检测抗双链ＤＮＡ抗体。 62nucleotide chain was synthesized,in the middle of which were 25 randomly synthesized nucleotides.Through PCR amplification a pool of DNA fragments containing myriads of variations was created.Positive patient sera can precipitate specific DNA fragments,which can then be amplified by PCR and detected as specific bands in agarose electrophoresis.Of 40 SLE sera,the PCR method had a positive rate of 80%,while the Farr RIA had 35%,the difference being statistically significant(P<0.005).Of 40 nonSLE CTD sera,the PCR method had a positive rate of 7.5%,while the Farr RIA had 2.5%,the difference being statistically insignificant(P>0.25).The results demonstrated that the PCR method was far more sensitive than RIA.In40 blood donor sera,both methods gave negative results.

关 键 词：抗双链 DNA PCR 抗体 系统性红斑狼疮 

分 类 号：R593.241[医药卫生—内科学]