生物处理镉污染土壤及其酶活性研究  

Bioremediation of Cd contaminated soil and a study of its enzyme activity

作　　者：姜春晓[1] 孙红文[1] 孙铁珩[1,2] 张清敏[1] 张彦峰[1] 

机构地区：[1]南开大学环境科学与工程学院,环境污染过程与基准教育部重点实验室,天津300071 [2]中国科学院沈阳应用生态研究所,辽宁沈阳110016

出　　处：《环境污染与防治》2009年第2期42-46,共5页Environmental Pollution & Control

基　　金：天津市农村工作委员会资助重大国际合作项目(No.0604130)

摘　　要：以添加镉的天津农田土壤为对象,分别设定空白处理、添加诺沃肥处理及生物强化处理(添加1株紫外线诱变工程菌和诺沃肥),研究土壤在不同pH、温度和含水率条件下可提取有效态镉(E-Cd)、过氧化氢酶及脲酶活性的变化情况。结果表明,添加诺沃肥处理可以有效降低E-Cd含量,而生物强化处理可进一步促进镉的固定效果;最适合生物修复的环境条件为pH7.0、温度30℃和土壤含水率50%;添加诺沃肥处理和生物强化处理的土壤中过氧化氢酶和脲酶活性明显高于空白处理,说明生物修复后,土壤酶活性已经恢复,表明这2种处理能改善土壤结构和性能,提高土壤的肥力。Remediation of the Cd-spiked Tianjin agricultural soil samples with and without nutrient or bacterial supplement （soil amendment） was performed for a period of 90 days： the fermented organic fertilizer NovoGro （SN treatment） and the UV-mutated microorganism Bacillus subtilis 38 （SNB treatment） were employed as the soil amendments. The effects of soil pH, temperature and moisture contents （water-holding capacity or WHC） on the ex tractable cadmium （E-Cd） content of the final soil samples and the activity profiles of catalase and urease of the 3 soils during the remediation. The best remediation results were obtained by treatment performed at 30 ℃ , pH of 7.0 on soil containing 50% of moisture. The catalase and urease activities were both higher with the SN and SNB amendments of the base soil samples suggesting that the soil enzyme activities were mostly recovered. The bioremediation with the amendment improved soil structure, property, and fertility.

关 键 词：诺沃肥 生物强化 镉 酶活性 修复 

分 类 号：X53[环境科学与工程—环境工程]