银屑病患者皮损中端粒酶活性的测定  

Detection of Telomerase Activity in the Lesion of Psoriasis

作　　者：程浩[1] 茅晓红[1] 张行[2] 蔡心涵[2] 孙国均[1] 郑树[2] 

机构地区：[1]浙江医科大学附属第二医院皮肤科,杭州310009 [2]浙江医科大学肿瘤研究所

出　　处：《中华皮肤科杂志》1998年第2期72-74,共3页Chinese Journal of Dermatology

摘　　要：目的　探讨银屑病的端粒酶活性表达情况。方法　采用端粒重复序列扩增文件——酶标法 (TRAP- EL ISA)测定银屑病皮损中端粒酶活性。结果　银屑病皮损中有一定水平的端粒酶活性 ,但其水平显著低于肿瘤组织和细胞株。结论　端粒酶不仅可在恶性肿瘤表达 ,也可在一些非恶性皮肤病组织表达 ;并且认为银屑病皮损中端粒酶活性的增加可能与银屑病发病机理尤其是表皮细胞的持续过度分化有某种关联。Objective To investigate telomerase activity in patients with psoriasis. Methods The telomerase activity in the skin lesions of patients was detected by TRAP (Telomeric repeat amplification protocol)——ELISA which is based upon PCR amplification of the initial telomerase product and detected by ELISA. Results Telomerase activity was detected in the lesions of psoriatic patients, but the level of it was lower in comparison with that in tissues of malignant tumor and K562 cell line. Conclusion These findings support that the increase of telomerase activity may be linked to the pathogenesis of psoriasis and that the telomerase activity can be detected not only in malignant tumors but also in nonmalignant skin diseases.

关 键 词：银屑病 皮损组织 DNS 核苷酸 外转移酶 端粒酶 

分 类 号：R758.630.2[医药卫生—皮肤病学与性病学]