基于四重iTRAQ标记结合LC-MALDI-TOF-TOF方法分析雌二醇和它莫西芬对MCF-7乳腺癌细胞内蛋白质含量影响的研究  

Study on the Effects of 17β-Oestradiol and Tamoxifen on the Content of Intracellular Proteins by iTRAQ Labeling/LC-MALDI-TOF-TOF Analysis

作　　者：倪国新[1] 郭立海 朱镭 郭妍[1] 徐学敏[1,3] 林标扬[1] 刘建华[3] 李伟 

机构地区：[1]上海交通大学系统生物医学研究院,上海200240 [2]爱谱拜斯应用生物系统贸易上海有限公司,上海200040 [3]上海交通大学生命科学与技术学院,上海200240

出　　处：《分析测试学报》2008年第12期1283-1287,共5页Journal of Instrumental Analysis

基　　金：科技部重大基础研究计划资助项目(2006CB0D0100);863-中国高技术研究发展计划(2006AA02Z108)

摘　　要：应用体外iTRAQ标记结合LC-MALDI-TOF-TOF方法对雌二醇刺激、雌二醇与它莫西芬共同处理,将它莫西芬处理与正常培养的MCF-7乳腺癌细胞内蛋白质表达的差异进行了比较分析。结果表明:各实验组与对照组之间存在数种上调或下调1倍以上的差异表达蛋白质,雌二醇可显著改变74种蛋白质的含量,雌二醇与它莫西芬共同处理引起26种蛋白质水平的显著变化,它莫西芬引起18种蛋白质表达的明显改变。各组之间既有变化趋势相同的蛋白质,也有变化趋势相反的蛋白质。The differential expression of intracellular proteins from 17/3-oestradiol（E2） stimulated, E2 plus tamoxifen（TAM） treated or TAM treated MCF-7 breast cancer cells was analyzed and compared with that from regularly cultured MCF-7 cells by using iTRAQ labeling coupled with LC - MALDI- TOF-TOF strategy. Numerous proteins were discovered to be significantly upregulated or downregulat- ed by more than two folds. E2, E2 ＋ TAM or TAM altered the expression of 74, 26 and 18 proteins significantly. The comparison of the change trends of protein between experimental groups and control groups showed that some proteins change to the same direction while others change to reverse direction. This study provides some useful information for elucidating the molecular mechanisms for the effects of E2 and TAM on breast cancer cells on protein level.

关 键 词：iTRAQ标记 LC-MALDI-TOF-TOF 雌二醇 它莫西芬 

分 类 号：O657.63[理学—分析化学] Q51[理学—化学]