酒中甲醇的离子排斥色谱-脉冲安培法测定  

Determination of Methanol in Wine by Ion Exclusion Chromatography with Pulsed Amperometric Detection

作　　者：韩静[1,2] 梁立娜[1] 牟世芬[1] 蔡亚岐[1] 鲁毅强[2] 

机构地区：[1]中国科学院生态环境研究中心环境化学与生态毒理学国家重点实验室,北京100085 [2]北京科技大学化学系,北京100083

出　　处：《分析测试学报》2008年第12期1343-1346,共4页Journal of Instrumental Analysis

基　　金：国家重点基础研究计划(973项目)(2003CB415001)

摘　　要：建立了离子排斥色谱-脉冲安培法测定酒中甲醇的检测方法。方法使用IonPac ICE-AS6离子排斥柱,以45 mmol/L HClO4为淋洗液,脉冲安培法检测（工作电极为Pt电极）。甲醇的检出限为0.03 mg/L（50μL进样,3倍基线噪音）;在7.9～158 mg/L范围内方法具有良好的线性（r=0.9990）。79 mg/L甲醇标准连续进样9次,峰面积相对标准偏差（RSD）为2.82%。用该方法检测了多种酒样中的甲醇含量,其加标回收率为91%～104%。A new method was developed for determination of methanol in wine by ion exclusion chro- matography. The separation of the methanol was achieved on an IonPac ICE-AS6 column by using 45 mmol/L HClO4 as isocratic eluent with a flow rate of 1.0 mL/min. The detection was performed with pulsed amperometric detector on a Pt working electrode. The method showed a good linearity over the methanol concentration in range of 7.9 - 158 mg/L with a detection limit of 0. 03 mg/L （50 μL injection, S/N = 3 ）. The relative standard deviations（RSDs） of the peak area for 79 mg/L methanol was 2. 82% （n = 9）. The established method was applied for the determination of methanol concentration in wines with spiked recoveries ranging from 91% to 104%.

关 键 词：酒 甲醇 离子排斥色谱 脉冲安培 

分 类 号：O657.75[理学—分析化学] TQ223.121[理学—化学]