太行菊顶芽离体高效再生系的建立  

Estabalishment of High Efficient Regeneration in vitro from Shoot tip of Opisthopapus taihangensis

作　　者：王建博[1] 徐思明[1] 董鹏[1] 孙佳[1] 李学东[1] 

机构地区：[1]首都师范大学生命科学学院,北京100037

出　　处：《首都师范大学学报（自然科学版）》2008年第5期45-50,共6页Journal of Capital Normal University：Natural Science Edition

基　　金：首都师范大学学生处科研立项项目

摘　　要：采用太行菊茎尖组培快繁技术,建立了太行菊无菌顶芽离体试管苗再生体系.通过MS培养基和激素配比实验,筛选出太行菊组织培养与快速繁殖的最佳培养基配方.结果表明:最适宜的外植体是幼嫩太行菊植株的茎顶芽;诱导愈伤组织的最适培养基为:MS+NAA 0.2 mg/L+6-BA 2.0 mg/L;诱导丛生芽的最适培养基为:MS+NAA 0.2mg/L+6-BA 2.0 mg/L+IBA 0.1 mg/L、MS+NAA 0.2 mg/L+6-BA 1.0 mg/L;诱导试管苗生根的最适培养基为:1/2 MS+NAA 0.2mg/L;Ca2+可明显提高芽的再生率;电子显微镜进行病毒检测后,筛选出3个脱病毒株系可提供优质种苗的种源.The shoot tip of Opisthopapus taihangensis were used as explants to produce plantlets in vitro.A virus-free regeneration system from shoot tip of Opisthopapus taihangensis was developed.A series of optimization experiment for cultural medium composition and concentration of phytohormones were investigated with a view to accelerate the propagation of virus-free materials.The results indicated that the most suitable explant was the tender shoot tip.It was shown that MS medium supplemented with NAA 0.2 mg/L＋6-BA 2.0 mg/L was good for callus induction,while MS medium containing NAA 0.2 mg/L＋6-BA 2.0 mg/L＋IBA 0.1 mg/L,NAA 0.2 mg/L＋6-BA（KT） 1.0 mg/L,half-strength MS medium containing NAA 0.2 mg/L gave best result for shoot induction and for rooting,respectively. Ca^2＋ enhanced shoot induction rate.Each tissue piece is suitable at the size of 20-25 mm^2.Virus-free materials were found repeatedly under electron microscope and 3 Virus-free strains were selected,which could be used as the source for rapid-propagation.

关 键 词：太行菊 快速繁殖 愈伤组织 土壤分析 病毒检测 

分 类 号：Q949.783.5[生物学—植物学]