Cu^(2+)和Zn^(2+)抗性真菌的分离、鉴定及其富集特性  

Isolation,identification and its bioaccumulation characteristics of a fungus strain with resistance to Cu^(2+) and Zn^(2+)

作　　者：刘云国[1] 樊霆[1] 周娜[1] 何义超[1] 闵忠义 邬思丹[1] 

机构地区：[1]湖南大学环境科学与工程学院,湖南长沙410082

出　　处：《中南大学学报（自然科学版）》2009年第1期60-66,共7页Journal of Central South University:Science and Technology

基　　金："十一五"国家科技支撑计划项目(2006BAD03A1704;2006BAD03A1706)

摘　　要：从湖南省岳阳市临湘铜锌尾砂坝土壤内分离、纯化得到1株抗铜和锌的菌株CTB430-1,分析该菌株形态和测定菌株ITS基因序列鉴定菌种,研究不同pH值、金属离子初始浓度和金属离子共存对菌体生长量和富集重金属的影响;分别采用考马斯亮兰法和碘量法测定菌体内可溶性蛋白和还原性谷胱甘肽含量。研究结果表明:鉴定该菌株为Aspergillus flavus,其对Cu2+和Zn2+的最低抑菌质量浓度分别为400和800mg/L;菌株的生长和富集受溶液pH值、金属离子初始质量浓度和金属离子共存的影响,Cu2+和Zn2+的富集量分别在其质量浓度为200和250mg/L时达到最大,为30.82和40.37mg/g;菌体内可溶性蛋白和还原性谷胱甘肽含量发生变化,表明菌株对Zn2+具有较强的适应能力和抗性,还原性谷胱甘肽能缓解Cu2+和Zn2+对CTB430-1的氧化损伤。A fungus CTB430-1 with resistance to copper and zinc was isolated from soil at a copper and Zinc Tailing of Linxiang in Yueyang city, Hunan province. The fungus was identified by analyzing the morphology and measuring the ITS sequence. The effects of pH, initial concentration, co-ions on the growth of CTB430-1 and bioaccumulation of Cu^2＋ and Zn^2＋ were studied. The protein and glutathione in the cells were determined by using the coomassie brilliant blue method and iodometric method, respectively. The results show that the strain is identified as Aspergillus flavus. Its minimal inhibitory concentration for Cu^2＋ and Zn^2＋are 400 and 800 rag/L, respectively, pH, initial concentration, co-ions in solution can affect the growth of CTB430-1 and bioaccumulation of Cu^2＋ and Zn^2＋, and the maximum bioaccumulation capacities of Cu^2＋ and Zn^2＋ are 30.82 and 40.37 mg/g at initial concentrations of 200 and 250 mg/L, respectively. Furthermore, the changes of protein and glutathione in the cells show that the strain holds high adaptive abilities and tolerance of zinc, and glutathione can alleviate the oxidation stress of copper and zinc.

关 键 词：真菌 铜 锌 抗性 富集 蛋白质 还原性谷胱甘肽 

分 类 号：X51[环境科学与工程—环境工程]