左旋精氨酸恢复肺动脉高压一氧化氮和氧自由基的平衡  

L-Arginine Restores Balance between Nitric Oxide and Superoxide Anion in Pulmonary Hypertension

作　　者：魏玮[1] 区景松[1] 黄达德[1] 罗炜[2] 罗兆榴[1] 欧志君[3] 陈文广[1] 邓卫兵[1] 

机构地区：[1]广州医学院附属广州市第一人民医院心胸外科∥广州市临床医学研究所,广东广州510180 [2]广州医学院附属第一医院∥广州呼吸疾病研究所,广东广州510120 [3]广州医学院广州市第一人民医院老年病科心血管专科,广东广州510180

出　　处：《中山大学学报（医学科学版）》2009年第1期43-46,共4页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：广东省医药卫生科技基金(A2005302);广州医学院科研启动基金(03-G-06)

摘　　要：【目的】探讨左旋精氨酸(l-Arg)对大鼠中期肺动脉高压(PH)的防治作用及机理。【方法】将18只雄性SD大鼠随机分为3组,对照组(C组,n=6)、PH组(M组,n=6)、l-Arg+PH组(L组,n=6)。于M组和L组用野百合碱(MCT)一次腹膜腔注射诱导PH模型,L组每天进行腹膜腔注射l-Arg。21d后用右心导管法测定右心室收缩压(RVSP);用化学比色法检测血浆中一氧化氮(NO)水平;用Hydroethidine进行肺灌注测量肺组织氧自由基(O2誗-)的产量。【结果】M组RVSP(31±5)mmHg明显高于C组(12±5)mmHg,而L组(23±5)mmHg比M组有所下降。血浆NO水平M组(11±7)μmol/L比C组(24±6)μmol/L低,而L组(22±12)μmol/L恢复到近C组水平。肺组织O2誗-含量M组(41±5)U明显高于C组(18.1±1.0)U,L组(27±3)U介于C组与M组之间。【结论】在PH中NO与O2誗-的平衡被打破,l-Arg可恢复PH中NO与O2誗-的平衡,这可能是L-Arg降低肺动脉压力的原因。[Objective] To investigate the role and the mechanism of L-Arginine （l-Arg） attenuating middle-stage pulmonary hypertension （PH）. [Methods] Eighteen healthy male SD rats were randomly divided into three groups. Saline was injected intraperitoneally （i.p.） in control group （C, n = 6）. Monoerotaline （MCT） was injected i.p. once in PH group （M, n = 6） to induce PH. MCT was injected i.p. once, followed by injecting l-Arg everyday for 21 d in PH ＋ l-Arg group （L, n = 6）. After 21 d, the right ventrieular systolic pressure （RVSP） was measured. The nitric oxide （NO） concentration in plasma was quantitatively assayed by chemical colorimetry. Superoxide anion （O2^.-） generation in lung was determined by perfusing lung with hydroethidine through pulmonary artery and analyzed by National Institutes of Health （NIH） Image J software. [Results] The RVSP was obviously higher in group M （31 ± 5） mmHg than in group C （12 ± 5） mmHg. But it partially decreased to （23 ± 5） mmHg in group L by being treated with l-Arg compared with group M. The plasma NO concentration was lower in group M （11 ± 7） μmol/ L than in group C （24 ± 6） μmol/L. However, l-Arg recovered the NO concentration to （22 ± 12）μmol/L in group L, which was not different from group C. The O2^.- generation in lung was higher in group M which was （41 ± 5） arbitrary unit than （18.1 ± 1.0） arbitrary unit in group C.l-Arg reduced the O2^.- generation to （27 ± 3） arbitrary unit in group L which lied between group C and group M. [Conelusion] The balance between NO and O2^.- was broken in PH. l-Arg can partially restore this balance which may be the reason why l-Arg inhibits PH.

关 键 词：肺动脉高压 一氧化氮 氧自由基 野百合碱 左旋精氨酸 

分 类 号：R543.2[医药卫生—心血管疾病]